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Please use this identifier to cite or link to this item: http://purl.org/purl/4083

Title: Potential application of β-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction
Authors: Bright Singh, I S
Prem, Gopinath
Mohandas, A
Divya, Jose
Jayesh, P
Keywords: β-1,3 glucanase
DNA extraction
Pseudomonas aeruginosa
Issue Date: 8-Aug-2013
Abstract: Pseudomonas aeruginosa MCCB 123 was grown in a synthetic medium for β-1,3 glucanase production. From the culture filtrate, β-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its β-1,3 glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and temperature for β-1,3 glucanase activity on laminarin was found to be 7 and 50 °C respectively. The MCCB 123 β-1,3 glucanase was found to have good lytic action on a wide range of fungal isolates, and hence its application in fungal DNA extraction was evaluated. β-1,3 glucanase purified from the culture supernatant of P. aeruginosa MCCB 123 could be used for the extraction of fungal DNA without the addition of any other reagents generally used. Optimum pH and temperature of enzyme for fungal DNA extraction was found to be 7 and 65 °C respectively. This is the first report on β-1,3 glucanase employed in fungal DNA extraction
Description: Indian Journal of Experimental Biology Vol.52, January 2014, pp. 89-96
URI: http://dyuthi.cusat.ac.in/purl/4083
Appears in Collections:Bright Singh I S

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