DSpace About DSpace Software

Dyuthi @ CUSAT >
Marine Biology, Micro Biology & Bio Chemistry >
Faculty >
Dr. A V Saramma >

Please use this identifier to cite or link to this item: http://purl.org/purl/4080

Title: Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture
Authors: Bright Singh, I S
Saramma, A V
Jayesh, P
Manjusha, K
Prem, Gopinath
Priyaja, P
Divya, Jose
Sreelakshmi, B
Keywords: Alkaline protease
Vibrio sp.
Animal cell culture
Primary cell culture
Virulence genes
Issue Date: 21-Jun-2012
Publisher: Springer
Abstract: Vibrio sp. V26 isolated from mangrove sediment showed 98 % similarity to 16S rRNA gene of Vibrio cholerae, V. mimicus, V. albensis and uncultured clones of Vibrio. Phenotypically also it resembled both V. cholerae and V. mimicus.Serogrouping, virulence associated gene profiling, hydrophobicity, and adherence pattern clearly pointed towards the non—toxigenic nature of Vibrio sp. V26. Purification and characterization of the enzyme revealed that it was moderately thermoactive, nonhemagglutinating alkaline metalloprotease with a molecular mass of 32 kDa. The application of alkaline protease from Vibrio sp. V26 (APV26) in sub culturing cell lines (HEp-2, HeLa and RTG-2) and dissociation of animal tissue (chick embryo) for primary cell culture were investigated. The time required for dissociation of cells as well as the viable cell yield obtained by while administeringAPV26 and trypsin were compared. Investigations revealed that the alkaline protease of Vibrio sp. V26 has the potential to be used in animal cell culture for subculturing cell lines and dissociation of animal tissue for the development of primary cell cultures, which has not been reported earlier among metalloproteases of Vibrios.
Description: Cytotechnology (2013) 65:199–212 DOI 10.1007/s10616-012-9472-z
URI: http://dyuthi.cusat.ac.in/purl/4080
Appears in Collections:Dr. A V Saramma

Files in This Item:

File Description SizeFormat
Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture.pdfpdf872.99 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback