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Please use this identifier to cite or link to this item: http://purl.org/purl/4058

Title: A new isoform of anti-lipopolysaccharide factor identified from the blue swimmer crab, Portunus pelagicus: Molecular characteristics and phylogeny
Authors: Bright Singh, I S
Rosamma, Philip
Sanjeevan, V N
Swapna, Antony P
Afsal, V V
Anil Kumar, P R
Keywords: Antimicrobial peptide
Portunus pelagicus
Anti-lipopolysaccharide factor
Crustacean immunity
Issue Date: 26-May-2012
Publisher: Elsevier
Abstract: Anti-lipopolysaccharide factors are small proteins that bind and neutralize lipopolysaccharide and exhibit potent antimicrobial activities. This study presents the molecular characterization and phylogenetic analysis of the first ALF isoform (Pp-ALF1; JQ745295) identified from the hemocytes of Portunus pelagicus. The full length cDNA of Pp-ALF1 consisted of 880 base pairs encoding 293 amino acids with an ORF of 123 amino acids and contains a putative signal peptide of 24 amino acids. Pp-ALF1 possessed a predicted molecular weight (MW) of 13.86 kDa and theoretical isoelectric point (pI) of 8.49. Two highly conserved cysteine residues and putative LPS binding domain were observed in Pp-ALF1. Peptide model of Pp-ALF1 consisted of two α-helices crowded against a four-strand β-sheet. Comparison of amino acid sequences and neighbor joining tree showed that Pp-ALF1 has a maximum similarity (46%) to ALF present in Portunus trituberculatus followed by 39% similarity to ALF of Eriocheir sinensis and 38% similarity to ALFs of Scylla paramamosain and Scylla serrata. Pp-ALF1 is found to be a new isoform of ALF family and its characteristic similarity with other known ALFs signifies its role in protection against invading pathogens.
Description: Aquaculture 356-357 (2012) 119–122
URI: http://dyuthi.cusat.ac.in/purl/4058
Appears in Collections:Bright Singh I S

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