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Please use this identifier to cite or link to this item: http://purl.org/purl/4051

Title: Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression
Authors: Bright Singh, I S
Jose, S
Jayesh, P
Sudheer, N S
Poulose, G
Mohandas, A
PhIlip, R
Keywords: BrdU assay
immune-related genes
immunofluorescence
lymphoid cell culture
Penaeus monodon
white spot syndrome virus genes
Issue Date: 1-May-2012
Publisher: Blackwell Publishing
Abstract: Shrimp cell lines are yet to be reported and this restricts the prospects of investigating the associated viral pathogens, especially white spot syndrome virus (WSSV). In this context, development of primary cell cultures from lymphoid organs was standardized. Poly-l-lysine-coated culture vessels enhanced growth of lymphoid cells, while the application of vertebrate growth factors did not, except insulin-like growth factor-1 (IGF-1). Susceptibility of the lymphoid cells to WSSV was confirmed by immunofluoresence assay using monoclonal antibody against the 28 kDa envelope protein of WSSV. Expression of viral and immunerelated genes in WSSV-infected lymphoid cultures could be demonstrated by RT-PCR. This emphasizes the utility of lymphoid primary cell culture as a platform for research in virus–cell interaction, virus morphogenesis, up and downregulation of shrimp immune-related genes, and also for the discovery of novel drugs to combat WSSV in shrimp culture
Description: Journal of Fish Diseases 2012, 35, 321–334 doi:10.1111/j.1365-2761.2012.01348.x
URI: http://dyuthi.cusat.ac.in/purl/4051
Appears in Collections:Bright Singh I S

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