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Please use this identifier to cite or link to this item: http://purl.org/purl/4047

Title: An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi
Authors: Bright Singh, I S
Rosamma, Philip
Jayaprakash, N S
Preetha, R
Sreedharan, Prathapan
Seena, Jose
Vijayan, K K
Keywords: antivibrio compound
N-methyl-1- hydroxyphenazine
liquid chromatography-mass spectroscopy
nuclear magnetic resonance spectroscopy
haemocyte culture
aquaculture
Issue Date: 1-Sep-2010
Publisher: Blackwell Publishing
Abstract: Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at di¡erent temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, puri¢ed and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UV^ Vis and nuclear magnetic resonance spectroscopy and identi¢ed as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC50 valuewas found to be1.4 0.31mg L 1. The compound was found to be bacteriostatic at 0.5mg L 1. Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application.
Description: Aquaculture Research, 2010, 41, 1452^1461 doi:10.1111/j.1365-2109.2009.02436.x
URI: http://dyuthi.cusat.ac.in/purl/4047
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