DSpace About DSpace Software

Dyuthi @ CUSAT >
Environmental Studies >
Faculty >
Bright Singh I S >

Please use this identifier to cite or link to this item: http://purl.org/purl/4038

Title: Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides
Authors: Bright Singh, I S
Seena, Jose
Rosamma, Philip
Mohandas, A
Jayesh, P
Keywords: Aquaculture
Penaeus monodon
Primary haemocyte culture
Heavy metals
Organophosphate pesticides
MTT assay Comet assay
Issue Date: 26-Dec-2010
Publisher: Elsevier
Abstract: Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos. Using MTT assay 12 h IC50 values calculated were 31.09 16.27 mM and 5.52 1.16 mM for cadmium chloride and mercuric chloride and 59.94 52.30 mg l 1 and 186.76 77.00 mg l 1 for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants
Description: Marine Environmental Research 71 (2011) 169-177
URI: http://dyuthi.cusat.ac.in/purl/4038
Appears in Collections:Bright Singh I S

Files in This Item:

File Description SizeFormat
Application of primary haemocyte culture of Penaeus monodon in the assessmentof cytotoxicity and genotoxicity of heavy metals and pesticides.pdfpdf855.83 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback