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Please use this identifier to cite or link to this item: http://purl.org/purl/4034

Title: Development of a cell culture system from the ovarian tissue of African catfish Clarias gariepinus
Authors: Bright Singh, I S
Sunil Kumar, G
Rosamma, Philip
Keywords: African catfish
Clarias gariepinus
Primary cell culture system
Issue Date: 11-Sep-2000
Publisher: Elsevier
Abstract: A growth medium with Leibovitz-15 L-15.as the base, supplemented with foetal bovine serum 10% vrv., fish muscle extract 10% vrv., prawn muscle extract 10% vrv., lectin concanavalin A. 0.02 mg mly1., lipopolysaccharide 0.02 mg mly1., glucose D 0.2 mg mly1., ovary extract 0.5% vrv.and prawn haemolymph 0.5%. has been formulated with 354"10 mOsm for the development and maintenance of a cell culture system from the ovarian tissue of African catfish, Clarias gariepinus. For its subculturing, a cell dissociationrextracting solution, composed of equal portions of trypsin phosphate versene glucose TPVG. containing 0.0125% wrv.trypsin and 25% vrv.non-enzymatic cell dissociation solution 1 and 2, has also been developed with which the cell culture can be passaged 15 times after which they cease to multiply and consequently perish. The cell cultures can be maintained for 12–15 days without fluid change between the passages. This is the first report of a cell culture system from the ovarian tissues of African catfish
Description: Aquaculture 194 (2001)51–62
URI: http://dyuthi.cusat.ac.in/purl/4034
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