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http://purl.org/purl/2312
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Title: | Invertase immobilised on montmorillonite: reusability enhancement and reduction in leaching |
Authors: | Sanjay, G Sugunan, S Science |
Keywords: | Immobilisation Immobilised enzymes Montmorillonite Adsorption Microporous Sucrose hydrolysis |
Issue Date: | 2005 |
Publisher: | Elsevier |
Abstract: | Invertase was immobilised on microporous montmorillonite K-10 via adsorption and covalent binding. The immobilised enzymes were tested for sucrose hydrolysis activity in a batch reactor. Km for immobilised systems was greater than free enzyme. The immobilised forms could be reused for 15 continuous cycles without any loss in activity. After 25 cycles, 85% initial activity was retained. A study on leaching of enzymes showed that 100% enzyme was retained even after 15 cycles of reuse. Leaching increased with reaction temperature. Covalent binding resisted leaching even at temperatures of 70 °C. |
URI: | http://dyuthi.cusat.ac.in/purl/2312 |
ISSN: | 1566-7367 |
Appears in Collections: | Dr. Sugunan S
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