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http://purl.org/purl/2261
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Title: | Enhanced pH and thermal stabilities of invertase immobilized on montmorillonite K-10 |
Authors: | Sanjay, G Sugunan, S Science |
Keywords: | Invertase Immobilization Montmorillonite Sucrose hydrolysis Adsorption Covalent binding |
Issue Date: | Mar-2006 |
Publisher: | Elsevier |
Abstract: | Invertase was adsorbed onto micro-porous acid-activated montmorillonite clay (K-10) by two procedures, namely adsorption and covalent binding. The immobilized enzymes were characterized by XRD, surface area measurements and 27Al NMR. XRD measurements revealed an expansion of clay layers due to immobilization which suggests that intercalation had taken place. Surface area measurements also support this observation. 27Al NMR showed that interaction of enzyme with tetrahedral and octahedral Al changes with the immobilization procedure. Sucrose hydrolysis was performed in a batch reactor. The immobilized enzymes showed enhanced pH and thermal stabilities. Optimum pH and temperature were found to increase upon immobilization. The effectiveness factor (η) and Michaelis constant (Km) suggest that diffusional resistances play a major role in the reaction. The immobilized invertase could be stored in buffer of pH 5 and 6 at 5 °C without any significant loss in activity for 20 days. |
URI: | http://dyuthi.cusat.ac.in/purl/2261 |
ISSN: | 0308-8146 |
Appears in Collections: | Dr. Sugunan S
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