Sarita,G Bhat; Jasmin, C; Sreeja, Chellappan; Rajeev, K Sukumaran; Elyas, K K; Chandrasekaran, M(Springer, January 10, 2010)
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Abstract:
An alkaline protease gene (Eap) was isolated
for the first time from a marine fungus, Engyodontium
album. Eap consists of an open reading frame of 1,161 bp
encoding a prepropeptide consisting of 387 amino acids
with a calculated molecular mass of 40.923 kDa. Homology
comparison of the deduced amino acid sequence of
Eap with other known proteins indicated that Eap encode
an extracellular protease that belongs to the subtilase
family of serine protease (Family S8). A comparative
homology model of the Engyodontium album protease
(EAP) was developed using the crystal structure of proteinase
K. The model revealed that EAP has broad substrate
specificity similar to Proteinase K with preference for
bulky hydrophobic residues at P1 and P4. Also, EAP is
suggested to have two disulfide bonds and more than two
Ca2? binding sites in its 3D structure; both of which are
assumed to contribute to the thermostable nature of the
protein.
Description:
World J Microbiol Biotechnol (2010) 26:1269–1279
DOI 10.1007/s11274-009-0298-6
Sarita,G Bhat; Anna Dilfi, K Francis; Zeena, Hamza P; Thomas, Kurian(Taylor & Francis, May 20, 2009)
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Abstract:
Low-density polyethylene was mixed with dextrin having
different particle sizes (100, 200 and 300 mesh). Various compositions
were prepared and their mechanical properties were evaluated
and thermal studies have been carried out. Biodegradability of these
samples has been checked using liquid culture medium containing
Vibrios (an amylase producing bacteria), which were isolated from
marine benthic environment. Soil burial test was done and reprocessability
of these samples was evaluated. The results indicate that the
newly prepared blends are reprocessable without sacrificing much of
their mechanical properties. The biodegradability tests on these
blends indicate that these are partially biodegradable
Description:
Polymer-Plastics Technology and Engineering, 48: 602–606, 2009
Sarita,G Bhat; Zeena, Hamza P; Anna Dilfi, K F; Thomas, Kurian(Taylor & Francis, November 24, 2008)
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Abstract:
Low-density polyethylene, (LDPE) was mixed with two grades of tapioca starch–lowgrade
and high-grade. Various compositions were prepared and mechanical and
thermal studies performed. The biodegradability of these samples was checked
using a culture medium containing Vibrios (an amylase-producing bacteria), which
was isolated from a marine benthic environment. The soil burial test and reprocessability
of these samples were checked. The studies on biodegradability show that
these blends are partially biodegradable. These low-density polyethylene-starch
blends are reprocessable without sacrificing much of their mechanical properties
Description:
International Journal of Polymeric Materials, 58:257–266, 2009