Saleena, Mathew; Maya, Raman(Springer, March 14, 2012)
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Abstract:
The chemical composition and evaluation of Indian
squid (Loligo duvauceli) mantle, epidermal connective
tissue and tentacle is investigated in this current study. It is
observed that squid mantle contains 22.2% total protein;
63.5% of the total protein is myofibrillar protein. The unique
property of squid myofibrillar protein is its water solubility.
Squid mantle contains 12.0% total collagen. Epidermal connective
tissue has highest amounts of total collagen (17.8%).
SDS-PAGE of total collagen identified high molecular
weight α-, β- and γ- sub-chains. Amino acid profile analysis
indicates that mantle and tentacle contain essential
amino acids. Arginine forms a major portion of mantle
collagen (272.5 g/100 g N). Isoleucine, glutamic acid and
lysine are other amino acids that are found in significantly
high amounts in the mantle. Sulphur containing cystine is
deficit in mantle collagen. Papain digest of mantle and
epidermal connective tissue is rich in uronic acid, while
papain digest, collagenase digest and urea digest of epidermal
connective tissue has significant amounts of sialic acid
(25.2, 33.2 and 99.8 μmol /100 g, respectively). PAS staining
of papain digest, collagenase digest and urea digest also
identify the association of hexoses with low molecular
weight collagen fragments. Histochemical sectioning also
emphasized the localized distribution of collagen in epidermal
and dermal region and very sparse fibres traverse the
myotome bundles