| dc.description.abstract |
Vibrio sp. V26 isolated from mangrove
sediment showed 98 % similarity to 16S rRNA gene
of Vibrio cholerae, V. mimicus, V. albensis and uncultured
clones of Vibrio. Phenotypically also it resembled
both V. cholerae and V. mimicus.Serogrouping, virulence
associated gene profiling, hydrophobicity, and adherence
pattern clearly pointed towards the non—toxigenic
nature of Vibrio sp. V26. Purification and characterization
of the enzyme revealed that it was moderately
thermoactive, nonhemagglutinating alkaline metalloprotease
with a molecular mass of 32 kDa. The application
of alkaline protease from Vibrio sp. V26 (APV26) in sub
culturing cell lines (HEp-2, HeLa and RTG-2) and
dissociation of animal tissue (chick embryo) for primary
cell culture were investigated. The time required for
dissociation of cells as well as the viable cell yield
obtained by while administeringAPV26 and trypsin were
compared. Investigations revealed that the alkaline
protease of Vibrio sp. V26 has the potential to be used
in animal cell culture for subculturing cell lines and
dissociation of animal tissue for the development of
primary cell cultures, which has not been reported earlier
among metalloproteases of Vibrios. |
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