Kinetic Studies of Purified malate Dehydrogenase in Liver of Streptozotocindiabetic Rats and the Effect of Leaf Extract of Aegle Marmelose (L.) Correa ex Roxb

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Kinetic Studies of Purified malate Dehydrogenase in Liver of Streptozotocindiabetic Rats and the Effect of Leaf Extract of Aegle Marmelose (L.) Correa ex Roxb

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dc.contributor.author Seema,P V
dc.contributor.author Sudha,B
dc.contributor.author Pius S,Padayatti
dc.contributor.author Asha,Abraham
dc.contributor.author Raghu,K G
dc.contributor.author Paulose,C S
dc.date.accessioned 2008-08-04T09:55:33Z
dc.date.available 2008-08-04T09:55:33Z
dc.date.issued 1996-06
dc.identifier.uri http://dyuthi.cusat.ac.in/purl/588
dc.description.abstract The functional basis of diabetes-mellitus to a certain extent, can be elucidated by studying diabetes-induced changes in metabolic enzymes. Malate dehydrogenase (MDH), is an enzyme directly involved in glucose metabolism. The kinetic parameters of MDH and its purified cytosolic isozyme, S-MDH, have been studied in the liver of streptozotocin- diabetic rats; also the potential of the leaf extract of A. marmelose as an was investigated. The Km of the liver enzyme increased significantly, in both crude and purified preparations in the diabetic state when compared to Lhe respective controls. Insulin as well as leaf- •extract treatment of the diabetic rats brought about a reversal of K. values to near normal. Vmax of purified S-MDH was significantly higher in the diabetic state when compared to the control. Insulin and leaf extract treatment did not reverse this change. Since MDH is an important enzyme in glucose metabolism, the variation in its quantitative and qualitative nature may contribute to the pathological status of diabetes. The fact that leaf extract of A. marmelose was found to be as effective as insulin in restoration of blood glucose and body weight to normal levels, the use of A. marmelose as potential hypoglycemic agent is suggested. en_US
dc.language.iso en en_US
dc.publisher Department of Biotecnology en_US
dc.subject Malate dehydrogenase (MDH) en_US
dc.subject Aegle marmelose en_US
dc.subject Glucose metabolism en_US
dc.subject Diabetes-mellitus en_US
dc.subject Cytosolic isozyme en_US
dc.subject Streptozotocin en_US
dc.subject Anti-diabetic agent en_US
dc.title Kinetic Studies of Purified malate Dehydrogenase in Liver of Streptozotocindiabetic Rats and the Effect of Leaf Extract of Aegle Marmelose (L.) Correa ex Roxb en_US
dc.type Working Paper en_US


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