Now showing items 1-8 of 8
| Abstract: | Aquaculture farms, particularly in Southeast Asia are facing severe crisis due to increasing incidences of White Spot Syndrome Virus (WSSV). Actinomycetes have provided many important bioactive compounds of high prophylactic and therapeutic value and are continually being screened for new compounds. In this communication, the results of a study made to determine the effectiveness of marine actinomycetes against the white spot disease in penaeid shrimps are presented. Twenty-five isolates of actinomycetes were tested for their ability to reduce infection due to WSSV among cultured shrimps. When these actinomycetes were made available as feed additives to the post-larvae of the black tiger shrimp Penaeus monodon for two weeks and challenged with WSSV, the post challenge survival showed variations from 11 to 83%. However, six isolates have shown to be the most potential candidates for further study. |
| URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2037 |
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| Antiviral property of marine actinomycetes.....pdf | (85.14Kb) |
| Abstract: | Budidaya menghasilkan sejumlah besar limbah, terdiri dari metabolisme oleh-produk, makanan sisa, materi feses dan residu dari masukan profilaksis dan terapeutik, mengarah pada penurunan kualitas air dan wabah penyakit. Bioremediasi, aplikasi mikroba / enzim untuk tambak, adalah metode yang sedang digunakan untuk meningkatkan kualitas air dan menjaga kesehatan dan stabilitas sistem akuakultur. Bioremediasi melibatkan mineralisasi bahan organik menjadi karbon dioksida, memaksimalkan produktivitas primer yang merangsang produksi udang, nitrifikasi dan denitrifikasi untuk (1) menghilangkan kelebihan nitrogen dari tambak dan (2) mempertahankan beragam dan stabil masyarakat kolam dimana patogen dikeluarkan dari sistem dan spesies diinginkan mendapatkan didirikan. Selain dari bahan organik merendahkan (detritivorous) bakteri heterotrofik, nitrifikasi, bakteri denitrifikasi dan fotosintesis umumnya digunakan dalam bioremediasi. |
| URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2032 |
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| Bioremediation in shrimp....pdf | (135.7Kb) |
| Abstract: | Aim: To develop a new medium for enhanced production of biomass of an aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine compound, pyocyanin. Methods and Results: Carbon and nitrogen sources and growth factors, such as amino acids and vitamins, were screened initially in a mineral medium for the biomass and antagonistic compound of Pseudomonas MCCB 103. The selected ingredients were further optimized using a full-factorial central composite design of the response surface methodology. The medium optimized as per the model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and the one optimized for the antagonistic compound contained mannitol (2 g l)1), glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral salts solution (20 ml l)1). Subsequently, the model was validated experimentally with a biomass increase by 19% and fivefold increase of the antagonistic compound. Conclusion: Significant increase in the biomass and antagonistic compound production could be obtained in the new media. Significance and Impact of the Study: Media formulation and optimization are the primary steps involved in bioprocess technology, an attempt not made so far in the production of aquaculture probiotics. |
| URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2034 |
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| Optimization of carbon and nitrogen....pdf | (662.4Kb) |
| Abstract: | A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to VIbrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L). |
| URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2036 |
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| Optimization of medium for the ....pdf | (479.9Kb) |
| Abstract: | Yeasts are ubiquitous in their distribution and populations mainly depend on the type and concentration of organic materials. The distribution of species, as well as their numbers and metabolic characteristics were found to be governed by existing environmental conditions. Marine yeasts were first discovered from the Atlantic Ocean and following this discovery, yeasts were isolated from different sources, viz. seawater, marine deposits, seaweeds, fish, marine mammals and sea birds. Nearshore environments are usually inhabited by tens to thousands of cells per litre of water, whereas low organic surface to deep-sea oceanic regions contain 10 or fewer cells/litre. Aerobic forms are found more in clean waters and fermentative forms in polluted waters. Yeasts are more abundant in silty muds than in sandy sediments. The isolation frequency of yeasts fell as the depth of the sampling site is increased. Major genera isolated in this study were Candida, Cryptococcus, Debaryomyces and Rhodotorula. For biomass estimation ergosterol method was used. Classification and identification of yeasts were performed using different criteria, i.e. morphology, sexual reproduction and physiological/biochemical characteristics. Fatty acid profiling or molecular sequencing of the IGS and ITS regions and 28S gene rDNA ensured accurate identification. |
| URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2035 |
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| Marine yeasts-a rewiew.pdf | (214.2Kb) |
| Abstract: | Soth west coast of india is blessed with a series of wetland systems popularly referred to as backwaters covering a total area of 46128.94 ha. these backwaters are internationallyrenowed for their aesthetic and scientific values including being a repository for several species fish and shell fishes. this is more significant in thatthre wetlands( Vembanad, Sasthamcotta & Ashtamudi) have recently been designated as Ramsar sites og international importance.Thirty major backwaters forming the crux of the coastal wetlands from an abodefor over 200 residentry and migratory fish and shellfish species. the fishing activities in these water bodies provide the livilihoodto about 200,000 fishers and also provide full time employment to over 50,000 fishermen. this paper describes the changes on the environmental and biodiversity status of selected wetlands, during 1994-2005 period. the pH was generally near neutral to alkaline in range. the salinity values indicated mixohaline condition ranging from 5.20-32.38 ppt in the 12 wetlands. the productivity values were generally low in mostof the wetlands during the study, where the gross production varied from 0.22gC/m3/ day in Kadinamkulam to 1.10gC/m3/day in the kayamkulam. the diversity of plankton and benthos was more during the pre monsoon compared to the monsoon and post monsoon periods in most of the wetlands.the diversity of plankton and benthos was more during the premonsoon compared to the monsoon and post monsoon periods in most of the wetlands.the average fish yield per ha.varied from 246kg in valapattanam to 2747kg in azhikode wetland. Retting of coconut husk in most of the wetlands led to acidic pH conditions with anoxia resulting in the production of high amounts of sulphide, coupled with high carbon dioxide values leading to drastic reduction in the incidence and abundance of plankton, benthic fauna and the fishery resources.The major fish species recorded from the investigation were Etroplus argentimaculatus, Mystus sp., tachysurus sp. and Hemiraamphus sp. . The majority of these back waters are highly stressed, especially during the pre monsoon period when the retting activity at its peak. The study has clearly reflected that a more restrained and cautions approach is needed to manage and preserve the unique backwater ecosystems of south west india. |
| URI: | http://hdl.handle.net/purl/1892 |
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| Bijoy S Nandan,.pdf | (916.1Kb) |
| URI: | http://dyuthi.cusat.ac.in/purl/2785 |
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| Dyuthi-P00384.pdf | (687.6Kb) |
| Abstract: | A crustinlike antimicrobial peptide from the haemocytes of giant tiger shrimp, Penaeus monodon was partially characterized at the molecular level and phylogenetic analysis was performed. The partial coding sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic of the shrimp crustins. Phylogenetic tree and sequence comparison clearly confirmed divergence of this crustinlike AMP from other shrimp crustins. The differential expression of the crustinlike AMP in P. monodon in response to the administration of various immunostimulants viz., two marine yeasts (Candida haemulonii S27 and Candida sake S165) and two bglucan isolates (extracted from C. haemulonii S27 and C. sake S165) were noted during the study. Responses to the application of two grampositive probiotic bacteria (Bacillus MCCB101 and Micrococcus MCCB104) were also observed. The immune profile was recorded preand postchallenge white spot syndrome virus (WSSV) by semiquantitative RTPCR. Expressions of seven WSSV genes were also observed for studying the intensity of viral infection in the experimental animals. The crustinlike AMP was found to be constitutively expressed in the animal and a significant downregulation could be noted postchallenge WSSV. Remarkable downregulation of the gene was observed in the immunostimulant fed animals prechallenge followed by a significant upregulation postchallenge WSSV. Tissuewise expression of crustinlike AMP on administration of C. haemulonii and Bacillus showed maximum transcripts in gill and intestine. The marine yeast, C. haemulonii and the probiotic bacteria, Bacillus were found to enhance the production of crustinlike AMP and confer significant protection to P. monodon against WSSV infection. |
| URI: | http://dyuthi.cusat.ac.in/purl/2784 |
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| Dyuthi-P00383.pdf | (840.4Kb) |
Now showing items 1-8 of 8
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