Bright Singh, I S; Lawman, D; Kutty, S N; Rosamma, Philip(May 14, 2013)
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Abstract:
Occurrence of black yeasts in the slope sediments of Bay of Bengal was investigated during FORV Sagar Sampada cruises 236 and 245. The black yeast population was found to be very scanty in the area and the isolates could be obtained from 200m to 1000m depth regions in the slope sediments. The isolates were identified as Hortaea werneckii by Internal Transcribed Spacer (ITS) sequencing. The biodegradation potential of these strains was found to be very high with all the strains exhibiting protease, lipase and amylase production. The optimum growth conditions were pH 8, salinity 30 ppt and temperature 30oC. The pigment melanin, in these organisms was identified to be of dihydroxynaphthalene type by NMR. The melanin was found to exhibit inhibitory activity against different human and fish pathogens. Melanin degrading enzyme could also be extracted from these organisms
Bright Singh, I S; Rosamma, Philip; Chaithanya, E R; Anil Kumar, P R; Sherine, Sonia Cubelio; Naveen, Sathyan(Springer, May 23, 2013)
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Abstract:
Hepcidin is a family of short cysteine-rich
antimicrobial peptides (AMPs) participating in various
physiological functions with inevitable role in host immune
responses. Present study deals with identification and
characterisation of a novel hepcidin isoform from coral fish
Zanclus cornutus. The 81 amino acid (aa) preprohepcidin
obtained from Z. cornutus consists of a hydrophobic aa rich
22 mer signal peptide, a highly variable proregion of 35 aa
and a bioactive mature peptide with 8 conserved cysteine
residues which contribute to the disulphide back bone. The
mature hepcidin, Zc-hepc1 has a theoretical isoelectric
point of 7.46, a predicted molecular weight of 2.43 kDa
and a net positive charge of ?1. Phylogenetic analysis
grouped Z. cornutus hepcidin with HAMP2 group hepcidins
confirming the divergent evolution of hepcidin-like
peptide in fishes. Zc-hepc1 can attain a b-hairpin-like
structure with two antiparallel b-sheets. This is the first
report of an AMP from the coral fish Z. cornutus.
Description:
Probiotics & Antimicro. Prot. (2013) 5:187–194
DOI 10.1007/s12602-013-9139-x
Pseudomonas aeruginosa MCCB 123 was grown in a synthetic medium for β-1,3 glucanase production. From the
culture filtrate, β-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its β-1,3
glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and temperature for β-1,3 glucanase activity on
laminarin was found to be 7 and 50 °C respectively. The MCCB 123 β-1,3 glucanase was found to have good lytic action on
a wide range of fungal isolates, and hence its application in fungal DNA extraction was evaluated. β-1,3 glucanase purified
from the culture supernatant of P. aeruginosa MCCB 123 could be used for the extraction of fungal DNA without the
addition of any other reagents generally used. Optimum pH and temperature of enzyme for fungal DNA extraction was
found to be 7 and 65 °C respectively. This is the first report on β-1,3 glucanase employed in fungal DNA extraction
Description:
Indian Journal of Experimental Biology
Vol.52, January 2014, pp. 89-96
Bright Singh, I S; Somnath Pai, S; Deepa, G D; Surekhamol, I S; Sreelakshmi, B; Varghese, S(The Society for Applied Microbiology, October 12, 2013)
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Abstract:
Of 33 phages isolated from various shrimp farms in Kerala, India, six were
segregated to have broad spectrum lytic efficiency towards 87 isolates of Vibrio
harveyi with cross-infecting potential to a few other important aquaculture
pathogens. They were further tested on beneficial aquaculture micro-organisms
such as probiotics and nitrifying bacterial consortia and proved to be
noninfective. Morphological characterization by transmission electron
microscopy (TEM) and molecular characterization by RAPD and SDS-PAGE
proved them distinct and positioned under Caudovirales belonging to
Myoviridae and Siphoviridae
Bright Singh, I S; Rosamma, Philip; Jayesh, P(Springer, February 25, 2014)
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Abstract:
Development of continuous cell lines from
shrimp is essential to investigate viral pathogens.
Unfortunately, there is no valid cell line developed
from crustaceans in general and shrimps in particular to
address this issue. Lack of information on the requirements
of cells in vitro limits the success of developing a
cell line, where the microenvironment of a cell culture,
provided by the growthmedium, is of prime importance.
Screening and optimization of growth medium components
based on statistical experimental designs have
been widely used for improving the efficacy of cell
culture media. Accordingly, we applied Plackett–Burman
design and response surface methodology to study
multifactorial interactions between the growth factors in
shrimp cell culture medium and to identify the most
important ones for growth of lymphoid cell culture from
Penaeus monodon. The statistical screening and
optimization indicated that insulin like growth factor-I
(IGF-I) and insulin like growth factor-II (IGF-II) at
concentrations of 100 and 150 ng ml-1, respectively,
could significantly influence the metabolic activity and
DNA synthesis of the lymphoid cells. An increase of
53 % metabolic activity and 24.8 % DNA synthesis
could be obtained, which suggested that IGF-I and IGFII
had critical roles in metabolic activity and DNA
synthesis of shrimp lymphoid cells