Bright Singh, I S; Vici, V; Sarita,G Bhat(Academic Press, March 10, 2000)
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Abstract:
The fresh water prawn, Macrobrachium rosenbergii, has proven potential for use as an
aquaculture species (Hanson & Goodwin, 1997; Kurup, 1984). In India alone, culture of
this species of prawn in low saline areas requires about 200 million seed per year
(Kurup, 1984). In hatcheries poor survival rate has been associated with vibriosis at
di#erent stages of the larval cycle. Members of the family Vibrionaceae associated with
the larvae of M. rosenbergii were shown to be pathogenic under laboratory conditions
(Bhat et al., 2000, in press). Vibrios have been associated with mortality of penaeid
prawns by several workers (Aquacop, 1977; Hameed, 1993; Karunasagar et al., 1994).
Two methods have been suggested to protect both the larvae and juveniles from
vibriosis; one is the administration of bacterins prepared from pathogenic strains
(Itami et al., 1989, 1991; Adams, 1991; Song & Sung, 1990; Sung et al., 1991) and the
other is the utilization of yeast 1-3 and 1-6 glucans as immunostimulants for
enhancing the non-specific defense system (Sung et al., 1994; Song et al., 1997). In the
light of these observations it was hypothesised that bacterins and yeast glucans may
also be e#ective in protecting the larvae of M. rosenbergii from vibriosis as has been
achieved in the case of penaeids. To examine this hypothesis, the ability of bacterins
and an extracellular glucan-producing yeast to increase the overall survival and
metamorphosis of larvae in a hatchery, as well as to protect against an experimental
challenge under laboratory conditions, was evaluated
Description:
Fish & Shellfish Immunology (2000) 10, 559–563
doi:10.1006/fsim.2000.0278
Bright Singh, I S; Jayaprakash, N S; Preetha, R; PhIlip, R(The Society for Applied Microbiology, July 20, 2006)
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Abstract:
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics
Description:
Journal of Applied Microbiology 102 (2007) 1043–1051
Bright Singh, I S; Preetha, R; Jayaprakash, N S(Inter-Research, March , 2007)
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Abstract:
Synechocystis MCCB 114 and 115 were segregated as putative probionts for shrimp
larvae from a collection of 54 cyanobacterial cultures enriched from seawater. On feeding Penaeus
monodon post-larvae with the cyanobacteria, the generic diversity of the intestinal bacterial flora
could be enhanced with substantial reduction or total absence of Vibrio spp. A significant difference
(p < 0.001) in the percent survival of batches of post-larvae fed on the cyanobacterial cultures was
observed and, on repeated challenge with V. harveyi, the relative percent survival of those batches
of larvae fed on Synechocystis MCCB 114 and 115 was significantly higher. The Synechocystis
MCCB 114 and 115 cultures were found to contain high levels of protein (34 to 43%), in addition to
carotenoids