Bright Singh, I S; Rosamma, Philip; Jayesh, P(Springer, February 25, 2014)
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Abstract:
Development of continuous cell lines from
shrimp is essential to investigate viral pathogens.
Unfortunately, there is no valid cell line developed
from crustaceans in general and shrimps in particular to
address this issue. Lack of information on the requirements
of cells in vitro limits the success of developing a
cell line, where the microenvironment of a cell culture,
provided by the growthmedium, is of prime importance.
Screening and optimization of growth medium components
based on statistical experimental designs have
been widely used for improving the efficacy of cell
culture media. Accordingly, we applied Plackett–Burman
design and response surface methodology to study
multifactorial interactions between the growth factors in
shrimp cell culture medium and to identify the most
important ones for growth of lymphoid cell culture from
Penaeus monodon. The statistical screening and
optimization indicated that insulin like growth factor-I
(IGF-I) and insulin like growth factor-II (IGF-II) at
concentrations of 100 and 150 ng ml-1, respectively,
could significantly influence the metabolic activity and
DNA synthesis of the lymphoid cells. An increase of
53 % metabolic activity and 24.8 % DNA synthesis
could be obtained, which suggested that IGF-I and IGFII
had critical roles in metabolic activity and DNA
synthesis of shrimp lymphoid cells