Balarama Kaimal,S; Gireesh,G; Paulose,C S(Department of Biotechnology, January 4, 2007)
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Abstract:
Gamma amino outyric acid is a major
inhibitory neurotrarsr titter in the central nervous
system. In the preset study sv, Have investigate(' the
alteration of GABA receptor, In t he hrain stem of rats
during pancreatic regeneration. Three groups of rats
were used for the study: sham operated, 72 It and
7 days partially pancreatectonnsea. GABA was (juan-
(ified by [H]GABA receptor iispiacement method.
GABA receptor kin: 10, pat at i et•ers were studied by
using the binding of F'.](iAhA as ligand to the Triton
X-100 treated me,i1,;-:mes a1,J displacement with
unlabelled GABA. GhRA,v receptor activity was
studied by using the [` -1 h3cuculline and displacement
with unlabellecV euculline. ;.\13A content significantly
decreased (1' < (1.(101 ) it, 0-e brain stern during
the regeneration of pancreas. 'I hl, high affinity (IAI3A
receptor binding sho?:ed it sigii'f cant decrease in 131„.,\
(P < 11.01) and K,I 1).05) n 72 h and 7 days after
partial pancreatee 'timv. ";:flhicuculline hin(Iing
showed it signih eat, 'le ( r(, :,e in /Jn1,s and K,I
(P < 0.001) in 72 h pa^.rcreaw,, mised rats when compared
with sham wt--tt' as P,n and K,I reversed to
near sham after 7 da,s of pancreatectomv. The results
sugge,) that GAB A throur,r; ('GABA receptors in
brain Atcem has a regulatory uie during active regeneration
of pancreas which will have inunense clinical
significance in the treatment of cliahetcs.
Paulose,C S; Amee,Krishnakumar; Anu,Joseph(Department of Biotechnology, 2007)
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Abstract:
Neuroscience is the study of'tbe ne rvous system , including the i - ; . in, spinal
cord and peripheral nerves . Neurons are the basic cells of the brain and
nervous system which exerts its functional role through various
neurotransmitters and receptor systems . The activity of a nen ren depends
on the balance between the number of excitatory and inhibito r y processes
affecting it, both processes occurring individually and sin ,tlte-' ,ieously. The
functional bal,ince of different neurotransmitters such as Acct >>lcholine
(Ach), Dopamine (DA), Serotonin (5-1-17), Nor epinepbri,te (N.1 j,
Epinephrine (LPI), Glutamate and Gamma amino butyric acid (GA BA)
regulates the growth , division and other vital functions ofa normal cell /
organisin (Sudha, 1 998). The micro-environ ; nertt of the cell is controlled
/ the macro-environment that surrounds the individual. Any change in
the cell environment causes imbalance in cell homeostasis and f,ntction.
Pollution is a significant cause of imbalance caused iii the inacYcenvironment.
Interaction with polluted environments can have an adverse
impact on the health of humans. The alarming rise in enviromilmieil
cont.iniin :rtion has been linked to rises in levels of pesticides, ndltstr al
effluents, domestic Waste, car exhausts and other anthropogenic activities.
Persistent exposures to contaminant cause a negative imp,-, on brain
health and development . Pollution also causes a change in the
neurotransmitters and their receptor function leading to earl.;' recurrence
of neurodcge,terative disorders such as flypoxia , Alzbeimers's and
Huntington 's disease early in life.
Optical fiber based laser induced fluorescence (LIF)
measurements were carried out using Rhodamine B to analyze
two different species of bacteria , a Gram-positive bacteria
namely Bacillus smithii , and fibrin alginolvticus, a Gram-
negative bacteria . The fiber sensor was clearly able to distinguish
between the two species of bacteria . Quenching effect of the dye
Rhodamine B by Bacillus smithii was observed . The effect of dye
on the samples was also studied in detail.