| Abstract: | Kinetic parameters of brain glutamate dehydrogenase (GDH) were compared in the brain stem, cerebellum and cerebral cortex of three weeks and one year old streptozotocin (STZ) induced four day diabetic rats with respective controls. A single intrafemoral dose of STZ (60mg/Kg body weight) was administered to induce diabetes in both age groups. After four days the blood glucose levels showed a significant increase in the diabetic animals of both age groups compared with the respective controls. The increase in blood glucose was significant in one year old compared to the three weeks old diabetic rats. The Vmm of the enzyme was decreased in all the brain regions studied, of the three weeks old diabetic rats without any significant change in the Km. In the adult the Vmax of GDH was increased in cerebellum and brain stem but was unchanged in the cerebral cortex. The K. was unchanged in cerebellum and cerebral cortex but was increased in the brain stem. These results suggest there may be an important regulatory role of the glutamate pathway in brain neural network disturbances and neuronal degeneration in diabetes as a function of age. |
| URI: | http://dyuthi.cusat.ac.in/purl/595 |
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| M P Biju and C S Paulose(1998) jan.PDF | (4.254Mb) |
| Abstract: | Cashew kernels have high nutritive value. Upon exposure to air kernels turn rancid and their nutritive value decreases. From this study it is concluded that chemical treatment using antioxidants reduced oxidative rancidity but failed to prevent deterioration in organoleptic characteristics and decrease in protein and carbohydrate content of stored kernels. |
| URI: | http://dyuthi.cusat.ac.in/purl/743 |
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| Rekha Ravindran and others,.PDF | (2.997Mb) |
| Abstract: | The role of thyroid hormones in DNA synthesis and in the activity of Thymidille kinase (TK), a key regulatory enzyme of DNA synthesis was studied in proliferating hepatocytes in vivo. Liver regeneration after partial hepatectomy was used as a model for controlled cell division in rats having different thyroid status - euthyroid, hypothyroid and 3,3',5'-triiodo-L-thyronine (T))-heated hypothyroid. Partial hepatectomy caused a significant elevation of DNA synthesis (p<0.01) in all the three groups compared to their sham-operated counterparts. Hypothyroid liepatectomised animals showed significantly lower (p<0.01) level of DNA synthesis than euthyroid hepatectomised animals. A single subcutaneous close of 1'3 to hypothyroid shamoperated animals resulted in a significant increase (p<0.01) of DNA synthesis in the intact liver. 17tis was comparable to the level of DNA synthesis occurring in regenerating liver of euthyroid animals. In hypothyroid hepatectomised animals, "1'3 showed an additive effect on l)NA synthesis and this group exhibited maximum level of DNA synthesis (p<0.0I ). Studies of the kinetic parameters of TK show that the Michelis-Menten constant, (K111) of TK for thymidine was altered by the thyroid status. K11 increased significantly (p<0.01) in untreated hypothyroid animals when compared to the euthyroid rats. '13 treatment of hypothyroid animals reversed this effect and this group showed the lowest value for K111 (p<0.01). Thus our results indicate that thyroid hormones can influence DNA synthesis during liver regeneration and they may regulate the activity of enzymes such as 17rymidine kinase which are important for DNA synthesis and hence cell division. |
| URI: | http://dyuthi.cusat.ac.in/purl/538 |
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| Tessy T Maliekal and others(19970feb.PDF | (5.704Mb) |
| Abstract: | Laser irradiation at wavelength 514 nm was used to study the effect, of lasers in inducing chromosomal aberrations at mitosis. This study offers a new radiation system which could be used for the induction of mutations. Results are compared with those obtained from studies using y-rays as irradiation source. |
| URI: | http://dyuthi.cusat.ac.in/purl/746 |
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| Unnikrishna Pil ... tion of Mitotic..,1997.PDF | (5.171Mb) |
| Abstract: | A comparative study of the effect of laser in inducing chromosomal aberrations at 488 nm was done in Vicia faba L. (faba bean) and Allium cepa L. (onion) with Argon ion laser (Spectra Physics Model 171). Seeds and bulbs of V. faba and A. cepa were subjected to laser irradiation by 488 nm excitations from Argon ion laser source at power levels 200 and 400 mW with power densities 2.25 mW cm-2 and 4.49 mW cm-2 and different exposure times ( 10, 20, 30 & 40 ..... tc) similar to the effect of other physical and chemical mutagens, laser caused a dose dependent decrease in mitotic index and a rise in mitotic aberrations when compared to the control . In both plant species, mutations were observed in all stages of mitotic cell cycle . The total percentage of aberrations was two fold higher at 400 mW than at 200 mW exposure. |
| URI: | http://dyuthi.cusat.ac.in/purl/747 |
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| Unnikrishna Pil ... on..Allium cepa L,1997.PDF | (3.611Mb) |
| Abstract: | The changes occuring to cashew kernels during storage at two humidity levels - 80% to 20% with respect to organoleptic characteristics, protein content, carbohydrate content, oil content, iodine and peroxide values were studied. From the present study it is concluded that organoleptic characteristics of cashew kernels deteriorates with increase in humidity. Decrease in protein and carbohydrate content of stored cashew kernel is dependent on humidity. Humidity increased oxidative rancidification. |
| URI: | http://dyuthi.cusat.ac.in/purl/732 |
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| Jisha K G and o ... in protein..,Aug.1997.PDF | (2.827Mb) |
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