Chandrasekaran, M; Madhu, K M; Beena, P S(Springer, 2009)
[+]
[-]
Abstract:
A potential fungal strain producing extracellular β-glucosidase enzyme was isolated from sea water and identified as ^ëéÉêJ
Öáääìë=ëóÇçïáá BTMFS 55 by a molecular approach based on 28S rDNA sequence homology which showed 93% identity
with already reported sequences of ^ëéÉêÖáääìë=ëóÇçïáá in the GenBank. A sequential optimization strategy was used to enhance
the production of β-glucosidase under solid state fermentation (SSF) with wheat bran (WB) as the growth medium.
The two-level Plackett-Burman (PB) design was implemented to screen medium components that influence β-glucosidase
production and among the 11 variables, moisture content, inoculums, and peptone were identified as the most significant
factors for β-glucosidase production. The enzyme was purified by (NH4)2SO4 precipitation followed by ion exchange chromatography
on DEAE sepharose. The enzyme was a monomeric protein with a molecular weight of ~95 kDa as determined
by SDS-PAGE. It was optimally active at pH 5.0 and 50°C. It showed high affinity towards éNPG and enzyme has a hã and
sã~ñ of 0.67 mM and 83.3 U/mL, respectively. The enzyme was tolerant to glucose inhibition with a há of 17 mM. Low concentration
of alcohols (10%), especially ethanol, could activate the enzyme. A considerable level of ethanol could produce from
wheat bran and rice straw after 48 and 24 h, respectively, with the help of p~ÅÅÜ~êçãóÅÉë=ÅÉêÉîáëá~É in presence of cellulase
and the purified β-glucosidase of ^ëéÉêÖáääìë=ëóÇçïáá BTMFS 55.
Description:
Biotechnology and Bioprocess Engineering 2009, 14: 457-466
DOI/10.1007/s12257-008-0116-2
Chandrasekaran, M; Suresh, P V(Elsevier, June 23, 1998)
[+]
[-]
Abstract:
A chitinolytic fungus, Beau6eria bassiana was isolated from marine sediment and significant process parameters influencing
chitinase production in solid state fermentation using wheat bran were optimised. The organism was strongly alkalophilic and
produced maximum chitinase at pH 9·20. The NaCl and colloidal chitin requirements varied with the type of moistening medium
used. Vegetative (mycelial) inoculum was more suitable than conidial inoculum for obtaining maximal enzyme yield. The addition
of phosphate and yeast extract resulted in enhancement of chitinase yield. After optimisation, the maximum enzyme yield was
246·6 units g 1 initial dry substrate (U gIDS 1). This is the first report of the production of chitinase from a marine fungus.