Paulose,C S; Dakshinamurti,K(Department of Biotechnology, June 12, 1987)
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Abstract:
Chronic catheterization is illustrated using vascular-access-port model SLA where the port is surgically placed subcutaneously on
the back of the rat. The catheter is tunnelled to the neck and inserted into the jugular vein . Within 24 h rats showed normal blood
pressure and blood samples were collected at intervals with minimal stress to the animals . A comparison of the plasma catecholamine
of blood collected from vascular-access-ports with that obtained from decapitation indicates that there was minimal stress to the rats
when blood was drawn through the vascular-access-port.
Paulose,C S; Dakshinamurti,K; Subah,Packer; Newman,L Stephens(Department of Biotechnology, December 8, 1987)
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Abstract:
Pyridoxal phosphate is the coenzyme of various decarboxylases involved in the formation
of monoamine neurotransmitters such as y-aminobutyric acid , serotonin , dopamine, and norepinephrine
. Adult male Sprague-Dawley rats placed on a pyridoxine -deficient diet for 8 weeks showed
significant hypertension compared with pyridoxine -supplemented controls . Hypothalamic contents of
pyridoxal phosphate , y-aminobutyric acid, and serotonin in the pyridoxine - deficient rats were significantly
lower than those in pyridoxine -supplemented controls . Hypertension was associated with
sympathetic stimulation . Treatment of pyridoxine-deficient rats with a single dose of pyridoxine (10
mg/kg body weight) reversed the blood pressure to normal levels within 24 hours, with concomitant
restorations of hypothalamic serotonin and y-aminobutyric acid as well as the return of plasma
norepinephrine and epinephrine to normal levels . Also, pyridoxine treatment reversed the hypothalamic
hypothyroidism observed in pyridoxine -deficient rats . These results indicate an association
between pyridoxine deficiency and sympathetic stimulation leading to hypertension.