| Abstract: | The morphological and biochemical response of calli and seedlings of different rice cultivars were compared under acid saline conditions. Calli of both tolerant and sensitive varieties showed severe stress symptoms like browning and necrosis, but the onset of stress symptoms was delayed in Pokkali. Seedlings of Pokkali showed minimal stress symptoms in lower salinities, and curling and senescence of older leaves in higher salinities although plants revived on amelioration of stress. Seedlings of the other varieties showed severe stress symptoms even at low salinities and plant death at higher salinities. Salt stress induced accumulation of the putative osmoprotectant proline in calli and seedlings of all varieties. Proline accumulation was higher in sensitive varieties than in Pokkali. These results indicate that proline accumulation is not directly correlated with salt tolerance in rice. |
| URI: | http://dyuthi.cusat.ac.in/purl/744 |
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| Renuka Devi,P S ... ,Prline accumulation...PDF | (1.866Mb) |
| Abstract: | Somatic embryos were induced from scutellar callus of immature zygotic embryos of T aestivum cv. Chinese Spring. Observations on precociously germinating somatic embryos revealed that: (i) In the initial stages the coleoptile is split, exposes the shoot apex and forms a green trichomatous leafy structure. In the germinating zygotic embryo, the coleoptile is tubular, (ii) Unlike what has been inferred earlier the leafy structure is the coleoptile and not the scutellum, (iii) Bipolarity of the embryoid is established later when root develops at the basal end. |
| URI: | http://dyuthi.cusat.ac.in/purl/740 |
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| Padma Nambisan ... bryogenesis...,Jan1992.PDF | (2.812Mb) |
| Abstract: | Plants were regenerated from callus induced from leaf disc explants of a tomato F, hybrid heterozygous for three marker loci (a), without anthocyanin (aw), and hairless (hl). Regenerants were studied for somaclonal variation at the phenotypic level by scoring for variation in the marker loci, and at the DNA level by probing geomic DNA blots with a chlorophyll a/b binding protein (Cab-3C) cDNA sequence. While no variation was observed at the phenotypic level in over 950 somaclones studied, DNA polymorphism for the Cab locus could be detected in two out of 17 somaclones tested. Tissue culture induced variation at the phenotypic level for specific loci is very low (less than 0.001 for a, awor hl) but DNA sequence changes are induced at much greater frequency (- 0.1 for a multicopy gene family such as Cab). |
| URI: | http://dyuthi.cusat.ac.in/purl/733 |
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| Padma nambisan ... polymorphism..,mar1992.PDF | (2.623Mb) |
| Abstract: | Alloxan induced diabetic animal model was used to evaluate the antidiabetic effect of alkaloids extracted from the leaves of Aegis marine/ose. The alkaloid extract maintained the weight of animals near to that of control ones - whereas there was a decrease in the body weight of diabetic animals. A significant increase in blood glucose (342. 14 -+- 14.89 mg/dl) was seen in diabetic animals but in alkaloid treated group the blood glucose was lowered (90: 12 +_5.81 mg/dl). There was no decrease in blood urea arid sreum cholesterol in the alkaloid treated group of diabetic animals. The liver glycogen decreased in diabetic animals (1.27+.12 g/100g of wet tissue) and the treatment brought the glycogen level to that of control ones (2.51 +.75 g/100 g of wet tissue). The result show that the alkaloid extract has hypoglycaemic activity. |
| URI: | http://dyuthi.cusat.ac.in/purl/585 |
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| P.T.C.Ponnachan and others(1993).PDF | (2.429Mb) |
| Abstract: | Alloxan induced animal model was used to evaluate the potential antidiabetic effect of A . marmelose leaf extract. The diabetic animals were given insulin injection and another group A . marmelose leaf extract orally. It maintained the weight of the animals near to the control rats but a significant decrease in weight was noted it diabetic animals without any treatment . The blood elucose level in 'treated animals were near to that of control ones. Also a significantly increased glucose tolerance was observed in animals orally given the leaf extract prior to the experiment . A significant decrease in liver glycogen ( 1.24 -.07 s!/ 100 g of wet tissue) was observed in diabetic rats which was brought to almost the normal level (I.84- . 14 g; 100 g) with Icaf extract treatment. Blood urea and serum cholesterol increased ( 62.66 - 3.50 and 192.67± 13.64 mg' dl) significantly in alloxxan diabetic rats . The leaf extract treatment decreased the blood urea and serum . cholesterol (37.83 - 3.97 and 99?0±8.43 mg/dl ) to that of control ones . A similar effect was seen with insulin treatment . The results indicate that the active principle in .4. marmelose leaf extract has similar hypoglycaemic activity to insulin treatment. |
| URI: | http://dyuthi.cusat.ac.in/purl/584 |
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| P.T.C.Ponnachan and others(1993)April.PDF | (2.230Mb) |
| Abstract: | Optical absorption characteristics of rat blood affected by diabetes has been studied using photoacoustic (PA) technique. PA spectrum of blood depends on the molecular structure of haemoglobin. The peak value ratio ylQ increases with increase in the diabetic state. Externally added glucose to normal blood does not show any increase in y//3 ratio as seen in the diabetic condition . The increase in yl,8 ratio may be due to the decrease in DPG level and the resultant shift from R -> T conformation of majority of diabetic haemoglobin. |
| URI: | http://dyuthi.cusat.ac.in/purl/539 |
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| T T Sreedevi and others (1994)may 24.PDF | (2.623Mb) |
| Abstract: | Response of callus culture and seedlings of a salt tolerant rice variety, pokkali, was compared to that of a salt sensitive variety, hrswa, under conditions of salinity stress induced by incorporating different quantities of sea water in the culture media. Seedlings of pokkali showed minimal symptoms of salt stress under conditions of low salinity and more severe symptoms including dry of older leaves under high salinity Conditions. However, seedlings of hrswa showed severe stress symptoms even under low salinity and plant death under high salinity conditions. This superior character of salt tolerance of pokkali over hrswa was, however, not observed in callus cultures. In both the cultivars, the severity of symptoms depended on the intensity of stress. Accumulation of free proline in callus cultures and seedlings under stress conditions was monitored. Both pokkali and hrswa accumulated proline in response to stress to the same level. The response was same in callus cultures and in seedlings of both cultivars and the rate of proline accumulation was dependent on the intensity of stress. This, study, thus, indicates that accumulation of proline is not indicative of salt tolerance character in rice. |
| URI: | http://dyuthi.cusat.ac.in/purl/745 |
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| Sabu, A and others,comparison of...,Feb 1995.PDF | (2.380Mb) |
| Abstract: | The functional basis of diabetes-mellitus to a certain extent, can be elucidated by studying diabetes-induced changes in metabolic enzymes. Malate dehydrogenase (MDH), is an enzyme directly involved in glucose metabolism. The kinetic parameters of MDH and its purified cytosolic isozyme, S-MDH, have been studied in the liver of streptozotocin- diabetic rats; also the potential of the leaf extract of A. marmelose as an was investigated. The Km of the liver enzyme increased significantly, in both crude and purified preparations in the diabetic state when compared to Lhe respective controls. Insulin as well as leaf- •extract treatment of the diabetic rats brought about a reversal of K. values to near normal. Vmax of purified S-MDH was significantly higher in the diabetic state when compared to the control. Insulin and leaf extract treatment did not reverse this change. Since MDH is an important enzyme in glucose metabolism, the variation in its quantitative and qualitative nature may contribute to the pathological status of diabetes. The fact that leaf extract of A. marmelose was found to be as effective as insulin in restoration of blood glucose and body weight to normal levels, the use of A. marmelose as potential hypoglycemic agent is suggested. |
| URI: | http://dyuthi.cusat.ac.in/purl/588 |
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| P V Seema and others (1996)june.PDF | (2.483Mb) |
| URI: | http://dyuthi.cusat.ac.in/purl/582 |
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| Preetha Nair and others(1996)oct.PDF | (2.515Mb) |
| Abstract: | The effect of insulin on cell proliferation in vivo has been studied in hepatectomised streptozotocin- diabetic rats. The extent of cell proliferation in sham and hepatectomized- control, diabetic and insulin treated rats were monitored by determining DNA content and [3H]thymidine incorporation into DNA. The kinetic parameters of thymidine kinase a regulatory enzyme for DNA synthesis was also studied in these groups. The rate of DNA synthesis in liver of streptozotocin -diabetic rats was significantly higher 24 hrs post-hepatectomy compared to control and insulin treated diabetic groups. Kinetic studies of thymidine kinase revealed that there was no change in the Michaelis -Menten constant (Km) whereas maximum velocity (Vmax) was elevated in the diabetic hepatectomized groups compared to control and insulin treated hepatectomized groups. Thus our study elucidates the role of insulin in thymidine kinase activity and DNA synthesis. |
| URI: | http://dyuthi.cusat.ac.in/purl/587 |
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| P Waliaula Mola and others(1996)nov.PDF | (6.602Mb) |
| Abstract: | In the present study we assessed plasma and platelet monoamine content using high performance liquid chromatography (HPLC). The study included 22 subjects consisting of 12 freshly-detected male diabetic patients and 10 age and sex-matched healthy controls. The same parameters were measured in streptozotocin -induced diabetic rat models consisting of controls , diabetic and insulin - treated diabetic rats. The platelet counts were significantly reduced (P < 0.05) in rat models as well as human diabetic samples. The plasma norepinephrine (NE) and epinephrine (EPI) concentrations were significantly increased (P < 0.05). The platelet showed a significant increase (P < 0.01) in NE, EPI and serotonin content. Increase in the plasma and platelet content of neurotransmitters may be due to increased sympathetic function, which is an adaptation for the decreased platelet count observed in our study . The results indicate that changes in the neurotransmitter content of the platelet may be a good index to assess the neurotransmitter status in pathological condition such as diabetes mellitus. |
| URI: | http://dyuthi.cusat.ac.in/purl/644 |
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| jakson James and others(1997)jan.PDF | (2.505Mb) |
| Abstract: | A comparative study of the effect of laser in inducing chromosomal aberrations at 488 nm was done in Vicia faba L. (faba bean) and Allium cepa L. (onion) with Argon ion laser (Spectra Physics Model 171). Seeds and bulbs of V. faba and A. cepa were subjected to laser irradiation by 488 nm excitations from Argon ion laser source at power levels 200 and 400 mW with power densities 2.25 mW cm-2 and 4.49 mW cm-2 and different exposure times ( 10, 20, 30 & 40 ..... tc) similar to the effect of other physical and chemical mutagens, laser caused a dose dependent decrease in mitotic index and a rise in mitotic aberrations when compared to the control . In both plant species, mutations were observed in all stages of mitotic cell cycle . The total percentage of aberrations was two fold higher at 400 mW than at 200 mW exposure. |
| URI: | http://dyuthi.cusat.ac.in/purl/747 |
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| Unnikrishna Pil ... on..Allium cepa L,1997.PDF | (3.611Mb) |
| Abstract: | Laser irradiation at wavelength 514 nm was used to study the effect, of lasers in inducing chromosomal aberrations at mitosis. This study offers a new radiation system which could be used for the induction of mutations. Results are compared with those obtained from studies using y-rays as irradiation source. |
| URI: | http://dyuthi.cusat.ac.in/purl/746 |
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| Unnikrishna Pil ... tion of Mitotic..,1997.PDF | (5.171Mb) |
| Abstract: | The role of thyroid hormones in DNA synthesis and in the activity of Thymidille kinase (TK), a key regulatory enzyme of DNA synthesis was studied in proliferating hepatocytes in vivo. Liver regeneration after partial hepatectomy was used as a model for controlled cell division in rats having different thyroid status - euthyroid, hypothyroid and 3,3',5'-triiodo-L-thyronine (T))-heated hypothyroid. Partial hepatectomy caused a significant elevation of DNA synthesis (p<0.01) in all the three groups compared to their sham-operated counterparts. Hypothyroid liepatectomised animals showed significantly lower (p<0.01) level of DNA synthesis than euthyroid hepatectomised animals. A single subcutaneous close of 1'3 to hypothyroid shamoperated animals resulted in a significant increase (p<0.01) of DNA synthesis in the intact liver. 17tis was comparable to the level of DNA synthesis occurring in regenerating liver of euthyroid animals. In hypothyroid hepatectomised animals, "1'3 showed an additive effect on l)NA synthesis and this group exhibited maximum level of DNA synthesis (p<0.0I ). Studies of the kinetic parameters of TK show that the Michelis-Menten constant, (K111) of TK for thymidine was altered by the thyroid status. K11 increased significantly (p<0.01) in untreated hypothyroid animals when compared to the euthyroid rats. '13 treatment of hypothyroid animals reversed this effect and this group showed the lowest value for K111 (p<0.01). Thus our results indicate that thyroid hormones can influence DNA synthesis during liver regeneration and they may regulate the activity of enzymes such as 17rymidine kinase which are important for DNA synthesis and hence cell division. |
| URI: | http://dyuthi.cusat.ac.in/purl/538 |
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| Tessy T Maliekal and others(19970feb.PDF | (5.704Mb) |
| Abstract: | The changes occuring to cashew kernels during storage at two humidity levels - 80% to 20% with respect to organoleptic characteristics, protein content, carbohydrate content, oil content, iodine and peroxide values were studied. From the present study it is concluded that organoleptic characteristics of cashew kernels deteriorates with increase in humidity. Decrease in protein and carbohydrate content of stored cashew kernel is dependent on humidity. Humidity increased oxidative rancidification. |
| URI: | http://dyuthi.cusat.ac.in/purl/732 |
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| Jisha K G and o ... in protein..,Aug.1997.PDF | (2.827Mb) |
| Abstract: | Kinetic parameters of brain glutamate dehydrogenase (GDH) were compared in the brain stem, cerebellum and cerebral cortex of three weeks and one year old streptozotocin (STZ) induced four day diabetic rats with respective controls. A single intrafemoral dose of STZ (60mg/Kg body weight) was administered to induce diabetes in both age groups. After four days the blood glucose levels showed a significant increase in the diabetic animals of both age groups compared with the respective controls. The increase in blood glucose was significant in one year old compared to the three weeks old diabetic rats. The Vmm of the enzyme was decreased in all the brain regions studied, of the three weeks old diabetic rats without any significant change in the Km. In the adult the Vmax of GDH was increased in cerebellum and brain stem but was unchanged in the cerebral cortex. The K. was unchanged in cerebellum and cerebral cortex but was increased in the brain stem. These results suggest there may be an important regulatory role of the glutamate pathway in brain neural network disturbances and neuronal degeneration in diabetes as a function of age. |
| URI: | http://dyuthi.cusat.ac.in/purl/595 |
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| M P Biju and C S Paulose(1998) jan.PDF | (4.254Mb) |
| Abstract: | Cashew kernels have high nutritive value. Upon exposure to air kernels turn rancid and their nutritive value decreases. From this study it is concluded that chemical treatment using antioxidants reduced oxidative rancidity but failed to prevent deterioration in organoleptic characteristics and decrease in protein and carbohydrate content of stored kernels. |
| URI: | http://dyuthi.cusat.ac.in/purl/743 |
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| Rekha Ravindran and others,.PDF | (2.997Mb) |
| URI: | http://dyuthi.cusat.ac.in/purl/576 |
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| Sudha Balasubramanian and C S P(1997)sept.PDF | (4.635Mb) |
| Abstract: | Effect of pyridoxine on growth, metabolism and cellular activity of freshwater prawn Macrobrachiuni rosenbergii was studied. Postlarvae (PL-10) of M. rosenbergii were fed with clam meat containing various concentrations of pyridoxine. After 30 days RNA and DNA of the abdominal tissues were estimated. Length, weight and RNA to DNA ratio increased significantly with increasing concentrations of pyridoxine. The effect of pyridoxine on the metabolic enzyme, malate dehydrogenase, was also studied. Vmax showed a significant decrease and the (Km) showed a significant increase in experimental groups compared to control. |
| URI: | http://dyuthi.cusat.ac.in/purl/596 |
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| K K Balachandran and others(1998)feb.PDF | (4.076Mb) |
| Abstract: | The use of laser excitations at two wavelengths, 488 rim and 514 nm, produced by Argon ion laser in two plants species, Vicia faba (faba bean) and Allium cepa L., is described to compare the mutagenicity and the spectrum of chromosomal aberrations induced by laser at mitosis. The laser irradiation has been done at two power levels 200 and 400 mW with power densities 2.25 mW cm 2 and 4.49 mW cm 2 and different exposure times (10, 20, 30 and 40 min). The effect produced by laser is compared to those of UV rays and y- radiations. Laser as in the case of other physical and chenucal mutagens causes a dose-dependent decrease in mitotic chromosomal aberrations at these wavelengths. It is postulated that laser could be used as a new radiation system for the induction of mutations. |
| URI: | http://dyuthi.cusat.ac.in/purl/748 |
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| Unnikrishna Pil ... rs as mutagens,may1998.PDF | (3.837Mb) |
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