Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

Dyuthi/Manakin Repository

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

Show simple item record

dc.contributor.author Swapna P, Antony
dc.contributor.author Bright Singh, I S
dc.contributor.author Sudheer, N S
dc.contributor.author Vrinda, S
dc.contributor.author Priyaja, P
dc.contributor.author Rosamma, Philip
dc.date.accessioned 2012-02-25T10:36:00Z
dc.date.available 2012-02-25T10:36:00Z
dc.date.issued 2011-01
dc.identifier.issn 01712985
dc.identifier.other Immunobiology 216 (2011) 184–194
dc.identifier.uri http://dyuthi.cusat.ac.in/purl/2784
dc.description.abstract A crustinlike antimicrobial peptide from the haemocytes of giant tiger shrimp, Penaeus monodon was partially characterized at the molecular level and phylogenetic analysis was performed. The partial coding sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic of the shrimp crustins. Phylogenetic tree and sequence comparison clearly confirmed divergence of this crustinlike AMP from other shrimp crustins. The differential expression of the crustinlike AMP in P. monodon in response to the administration of various immunostimulants viz., two marine yeasts (Candida haemulonii S27 and Candida sake S165) and two bglucan isolates (extracted from C. haemulonii S27 and C. sake S165) were noted during the study. Responses to the application of two grampositive probiotic bacteria (Bacillus MCCB101 and Micrococcus MCCB104) were also observed. The immune profile was recorded preand postchallenge white spot syndrome virus (WSSV) by semiquantitative RTPCR. Expressions of seven WSSV genes were also observed for studying the intensity of viral infection in the experimental animals. The crustinlike AMP was found to be constitutively expressed in the animal and a significant downregulation could be noted postchallenge WSSV. Remarkable downregulation of the gene was observed in the immunostimulant fed animals prechallenge followed by a significant upregulation postchallenge WSSV. Tissuewise expression of crustinlike AMP on administration of C. haemulonii and Bacillus showed maximum transcripts in gill and intestine. The marine yeast, C. haemulonii and the probiotic bacteria, Bacillus were found to enhance the production of crustinlike AMP and confer significant protection to P. monodon against WSSV infection. en_US
dc.description.sponsorship Cochin University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Antimicrobial Peptide en_US
dc.subject Crustin en_US
dc.subject Penaeus monodon en_US
dc.subject Immunostimulant en_US
dc.subject Yeast en_US
dc.subject beta-1,3 glucan en_US
dc.subject Probiotic en_US
dc.subject WSSV en_US
dc.title Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV en_US
dc.type Working Paper en_US


Files in this item

Files Size Format View Description
Dyuthi-P00383.pdf 820.7Kb PDF View/Open PDF

This item appears in the following Collection(s)

Show simple item record

Search Dyuthi


Advanced Search

Browse

My Account