Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Micrococcus MCCB 104 Using Central Composite Design

Dyuthi/Manakin Repository

Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Micrococcus MCCB 104 Using Central Composite Design

Show simple item record

dc.contributor.author Rosamma, Philip
dc.contributor.author Preetha, R
dc.contributor.author Jayaprakash, N S
dc.contributor.author Bright Singh, I S
dc.date.accessioned 2010-12-14T09:55:00Z
dc.date.available 2010-12-14T09:55:00Z
dc.date.issued 2007
dc.identifier.other Biotechnology and Bioprocess Engineering 2007, 12: 548-555
dc.identifier.uri http://dyuthi.cusat.ac.in/xmlui/purl/2036
dc.description.abstract A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to VIbrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L). en_US
dc.description.sponsorship Cochin University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher KSBB en_US
dc.subject Probiotic en_US
dc.subject Antagonism en_US
dc.subject Micrococcus en_US
dc.subject Response surface methodology en_US
dc.subject Central composite design en_US
dc.subject Aquaculture en_US
dc.title Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Micrococcus MCCB 104 Using Central Composite Design en_US
dc.type Working Paper en_US
dc.contributor.faculty Marine Sciences en_US


Files in this item

Files Size Format View
Optimization of medium for the ....pdf 468.6Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search Dyuthi


Advanced Search

Browse

My Account