Rosamma, Philip; Preetha, R; Jayaprakash, N S; Bright Singh, I S(KSBB, 2007)
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Abstract:
A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to VIbrio. In the
present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced
biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface
methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of
fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response
surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between
the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors
for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L),
ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L).
Rosamma, Philip; Preetha, R; Jayaprakash, N S; Bright Singh, I S(The Society for Applied Microbiology, 2007)
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Abstract:
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics.