Jayesh, P; Dr.Bright Singh,I S(Cochin University of Science and Technology, September , 2012)
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Abstract:
Unveiling the molecular and regulatory mechanisms that prevent in vitro
transformation in shrimp remains elusive in the development of continuous cell
lines, with an arduous history of over 25 years (Jayesh et al., 2012). Despite
presenting challenges to researchers in developing a cell line, the billion dollar
aquaculture industry is under viral threat. In addition, the regulatory mechanisms
that prevent in vitro transformation and carcinoma in shrimps might provide new
leads for the development of anti-ageing and anti-cancer interventions in human
(Vogt, 2011) and in higher vertebrates. This highlights the importance of
developing shrimp cell lines, to bring out effective prophylactics against shrimp
viruses and for understanding the mechanism that induce cancer and ageing in
human.. Advances in molecular biology and various gene transfer technologies for
immortalization of cells have resulted in the development of hundreds of cell lines
from insects and mammals, but yet not a single cell line has been developed from
shrimp and other marine invertebrates. With this backdrop, the research described
in this thesis attempted to develop molecular tools for induced in vitro
transformation in lymphoid cells from Penaeus monodon and for the development
of continuous cell lines using conventional and novel technologies to address the
problems at cellular and molecular level.
Description:
National Centre for Aquatic Animal
Health, School of Environmental Studies, Cochin University of Science and
Technology