Bright Singh, I S; Sunil Kumar, G; Rosamma, Philip(Elsevier, September 11, 2000)
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Abstract:
A growth medium with Leibovitz-15 L-15.as the base, supplemented with foetal bovine
serum 10% vrv., fish muscle extract 10% vrv., prawn muscle extract 10% vrv., lectin
concanavalin A. 0.02 mg mly1., lipopolysaccharide 0.02 mg mly1., glucose D 0.2 mg mly1.,
ovary extract 0.5% vrv.and prawn haemolymph 0.5%. has been formulated with 354"10
mOsm for the development and maintenance of a cell culture system from the ovarian tissue of
African catfish, Clarias gariepinus. For its subculturing, a cell dissociationrextracting solution,
composed of equal portions of trypsin phosphate versene glucose TPVG. containing 0.0125%
wrv.trypsin and 25% vrv.non-enzymatic cell dissociation solution 1 and 2, has also been
developed with which the cell culture can be passaged 15 times after which they cease to multiply
and consequently perish. The cell cultures can be maintained for 12–15 days without fluid change
between the passages. This is the first report of a cell culture system from the ovarian tissues of
African catfish