This study shows that the disease resistance and survival
rate of Penaeus monodon in a larval rearing systems
can be enhanced by supplementing with antagonistic
or non-antagonistic probiotics. The antagonistic mode
of action of Pseudomonas MCCB 102 and MCCB 103
against vibrios was demonstrated in larval mesocosm
with cultures having su⁄cient concentration of antagonistic
compounds in their culture supernatant. Investigations
on the antagonistic properties of Bacillus
MCCB 101, Pseudomonas MCCB 102 and MCCB 103
and Arthrobacter MCCB 104 against Vibrio harveyi
MCCB111under in vitro conditions revealed that Pseudomonas
MCCB 102 and MCCB 103 were inhibitory to
the pathogen.These inhibitory propertieswere further
con¢rmed in the larval rearing systems of P. monodon.
All these four probionts signi¢cantly improved larval
survival in long-term treatments as well as when challengedwith
a pathogenic strain ofV. harveyiMCCB111.
We could demonstrate that Pseudomonas MCCB 102
andMCCB103 accorded disease resistance and a higher
survival rate in P. monodon larval rearing systems
throughactive antagonism of vibrios,whereas Bacillus
MCCB 101 and Arthrobacter MCCB 104 functioned as
probiotics through immunostimulatory and digestive
enzyme-supporting modes of action.
Hatha, A A M; Mujeeb Rahiman, K M; Yousuf, Jesmi; Ambat,Thomas P(Blackwell Publishing Ltd, 2010)
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Abstract:
Eight hundred and eighty-¢ve strains of bacterial isolates
fromvarious samples associatedwith the natural
habitat ofMacrobrachiumrosenbergii were screened
for their probiotic potential. Two putative probionts
namely Bacillus NL110 and Vibrio NE17 isolated from
the larvae and egg samples, respectively, were selected
for experimental studies and were introduced
to the juveniles of M. rosenbergii (0.080 0.001g)
through di¡erent modes such as through feed, water
and both. The probiotic potential of the above bacteria
in terms of improvements inwater quality, growth,
survival, speci¢c growth rate (SGR), feed conversion
ratio and immune parameters was evaluated. The
treatment groups showed a signi¢cant improvement
in SGR and weight gain (Po0.001). Survival among
di¡erent treatment groups was better than that in
the control group. There were also signi¢cant improvements
in the water quality parameters such as
the concentration of nitrate and ammonia in the
treatment groups (Po0.05). Improvements in immune
parameters such as the total haemocyte count
(Po0.05), phenoloxidase activity and respiratory
burst were also signi¢cant (Po0.001). It is concluded
that screening of the natural micro£ora of cultured
¢sh and shell¢sh for putative probionts might yield
probiotic strains of bacteria that could be utilized for
an environment-friendly and organic mode of aquaculture.
Hatha, A A M; Mujeeb Rahiman, K M; Yousuf, Jesmi; Ambat,Thomas P(Blackwell Publishing Ltd, 2010)
[+]
[-]
Abstract:
Eight hundred and eighty-¢ve strains of bacterial isolates
fromvarious samples associatedwith the natural
habitat ofMacrobrachiumrosenbergii were screened
for their probiotic potential. Two putative probionts
namely Bacillus NL110 and Vibrio NE17 isolated from
the larvae and egg samples, respectively, were selected
for experimental studies and were introduced
to the juveniles of M. rosenbergii (0.080 0.001g)
through di¡erent modes such as through feed, water
and both. The probiotic potential of the above bacteria
in terms of improvements inwater quality, growth,
survival, speci¢c growth rate (SGR), feed conversion
ratio and immune parameters was evaluated. The
treatment groups showed a signi¢cant improvement
in SGR and weight gain (Po0.001). Survival among
di¡erent treatment groups was better than that in
the control group. There were also signi¢cant improvements
in the water quality parameters such as
the concentration of nitrate and ammonia in the
treatment groups (Po0.05). Improvements in immune
parameters such as the total haemocyte count
(Po0.05), phenoloxidase activity and respiratory
burst were also signi¢cant (Po0.001). It is concluded
that screening of the natural micro£ora of cultured
¢sh and shell¢sh for putative probionts might yield
probiotic strains of bacteria that could be utilized for
an environment-friendly and organic mode of aquaculture