Bright Singh, I S; Rosamma, Philip; Jayesh, P(Springer, February 25, 2014)
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Abstract:
Development of continuous cell lines from
shrimp is essential to investigate viral pathogens.
Unfortunately, there is no valid cell line developed
from crustaceans in general and shrimps in particular to
address this issue. Lack of information on the requirements
of cells in vitro limits the success of developing a
cell line, where the microenvironment of a cell culture,
provided by the growthmedium, is of prime importance.
Screening and optimization of growth medium components
based on statistical experimental designs have
been widely used for improving the efficacy of cell
culture media. Accordingly, we applied Plackett–Burman
design and response surface methodology to study
multifactorial interactions between the growth factors in
shrimp cell culture medium and to identify the most
important ones for growth of lymphoid cell culture from
Penaeus monodon. The statistical screening and
optimization indicated that insulin like growth factor-I
(IGF-I) and insulin like growth factor-II (IGF-II) at
concentrations of 100 and 150 ng ml-1, respectively,
could significantly influence the metabolic activity and
DNA synthesis of the lymphoid cells. An increase of
53 % metabolic activity and 24.8 % DNA synthesis
could be obtained, which suggested that IGF-I and IGFII
had critical roles in metabolic activity and DNA
synthesis of shrimp lymphoid cells
Rosamma, Philip; Preetha, R; Jayaprakash, N S; Bright Singh, I S(The Society for Applied Microbiology, 2007)
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Abstract:
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics.