Simi Joseph,P; Dr.Rosamma,Philip(Cochin University of Science & Technology, July , 2009)
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Abstract:
The main source of protein for human and animal consumption is from
the agricultural sector, where the production is vulnerable to diseases,
fluctuations in climatic conditions and deteriorating hydrological conditions due
to water pollution. Therefore Single Cell Protein (SCP) production has evolved
as an excellent alternative. Among all sources of microbial protein, yeast has
attained global acceptability and has been preferred for SCP production. The
screening and evaluation of nutritional and other culture variables of
microorganisms are very important in the development of a bioprocess for SCP
production. The application of statistical experimental design in bioprocess
development can result in improved product yields, reduced process variability,
closer confirmation of the output response to target requirements and reduced
development time and overall cost.The present work was undertaken to develop a bioprocess technology for
the mass production of a marine yeast, Candida sp.S27. Yeasts isolated from the
offshore waters of the South west coast of India and maintained in the
Microbiology Laboratory were subjected to various tests for the selection of a
potent strain for biomass production. The selected marine yeast was identified
based on ITS sequencing. Biochemical/nutritional characterization of Candida
sp.S27 was carried out. Using Response Surface Methodology (RSM) the
process parameters (pH, temperature and salinity) were optimized. For mass
production of yeast biomass, a chemically defined medium (Barnett and Ingram,
1955) and a crude medium (Molasses-Yeast extract) were optimized using
RSM. Scale up of biomass production was done in a Bench top Fermenter using
these two optimized media. Comparative efficacy of the defined and crude
media were estimated besides nutritional evaluation of the biomass developed
using these two optimized media.
Description:
Dept. of Marine Biology, Microbiology and
Biochemistry, Cochin University of Science and
Technology,