Manjusha, K; Dr.Saramma, A V(Cochin University of Science and Technology, July , 2011)
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Abstract:
The thesis presents a detailed account of the alkaline protease produced by Vibrio sp.(V26) a mangrove isolate,and the application of this enzyme in different fields.The protease producer strain was identified on the basis of biochemical characteristice,putative virulence traits and 16S rRNA gene sequencing.The purification and characterization of the protease has been carried out. Along with this, an attempt has been made to identifiy the protease gene. The physical parameters as well as the media components influencing protease production were optimized using Response Surfce Methodology(RSM).The scale up of the application of the protease from Vibrio sp.(V26) in the dissociation of cells in animal cell culture,in the recovery of silver from used X-ray films as well as an ingredient in commercial detergents were investigated.
Description:
Dept.of Marine Biology,Microbiology and Biochemistry,Cochin University of Science and Technology
Sindhu,O K; Dr.Surendran, P K(Central Institute of Fisheries Technology, January , 2007)
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Abstract:
A detailed study was made on the microbial quality, with special reference to food
safety, of the fish and fishery products in the retail trade in Cochin and around. Also,
farmed molluscan shellfishes like mussels and oysters were investigated for the microbial
quality including the presence of pathogenic bacteria. Special stress has been given to
monitor the incidence of coagulase positive as well as coagulase negative Staphylococcus
in these products and their relative incidence have been recorded.In the next part, the investigation was centered mainly on toxigenic S.aureus. This
is because among the Gram positive toxigenic bacteria, the Saureus with potential to
produce thermostable enterotoxins are more relavent in food safety conceming seafoods
in comparison with the Gram-negative pathogens like Salmonella and V.cholerae.The incidence, toxigenic potential and conditions of toxin production by S.aureus
have been investigated in detail. An attempt has also been made to relate the toxigenisis
with the presence of the concerned toxigenic genes in the genomes of S. aureus strains.
Lakshmi G,Nair; Dr.Rosamma,Philip(Cochin University of Science and Technology, May , 2008)
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Abstract:
The present study revealed the importance of marine actinomycetes as a potent source of
bio active secondary metabolites. The selected isolates were capable of protecting Peaneus
monodon against WSSV infection. They also proved to be inhibitory to vibrios and
is a rich pool of hydrolytic enzymes. Their capacity to proliferate in saline environments
and their property of non-pathogenicity to prawns makes them good candidates to be applied
as probionts in penaeid shrimp aquaculture. They also enhanced the immune status of
shrimps challenged with WSSV and act as a good source of antioxidants. Exploitation of
the potential for the prophylactic and therapeutic measures in aquatic animal health management
would be highly rewarding. This work is a preliminary study targeting marine
actinomycetes as a source of antiviral compounds and as probionts in Penaeus monodon
culture systems. More work is needed to understand the nature and mode of action of the
bioactive compound, the various aspects of immune and antioxidant responses under challenge
and when exposed to pro active treatments, and the dose and frequency of application
of such compounds under rearing conditions.
Description:
Department of Marine Biology, Microbiology & Biochemistry,Cochin University of Science & Technology