Shylaraj, K S; Dr.Chandrasekaran,M(Cochin University of Science And Technology, August 27, 1998)
[+]
[-]
Abstract:
In the light of the very huge demand for natural ephedrine and
pseudoephidrine, a search for an angiosperm plant containing the alkaloid ephedrine
was made and could locate Sida spp. of malvaceae family. Sida is a large genus of,
herbs and shrubs distributed throughout the tropics. About a dozen species occur in
India. The medicinally important species known are S.rhombrfolia S.cordata and
S.spinosa (Anon, 1972). Among the various species, S.rh0mbIfolia is the most widely
used one in the traditional system of medicine. An attempt was made in the present
study to develop an ideal bioprocess for the in vitro production of ephedrine from the
cell culture system of Sida rhombrfolia Linn. ssp. retusa. The callus and suspension
culture were initiated and attempts were made to enhance the yield positively by
employing various strategies like mutagenesis, immobilization and addition of
precursors, elicitors and penneabilizing agents.
Description:
Department of Biotechnology
Cochin University of Science and Technology