Bijina, B; Dr.Chandrasekaran,M(Cochin University of Science & Technology, May , 2006)
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Abstract:
Protease inhibitors are one of the most important tools of nature for
regulating the proteolytic activity of their target proteases. They are
synthesized in biological systems and they play a critical role in controlling
a number of diverse physiological functions. The current investigation
focused on the isolation, purification and characterization of a novel
protease inhibitor from Moringa oleifera. The results obtained during the
course of study opens new perspectives for the utilization of protease
inhibitor from Moringa oleifera for various pharmaceutical, agricultural and
food industries. The biological and physicochemical properties exhibited by
the novel protease inhibitor from Moringa oleifera clearly testify its
suitability for the development as a drug for application in pharmaceutical
industries such as anticoagulant agent or biocontrol agent in agriculture and
even as a food preservant. There is a scope for further research on the
structure elucidation and protein engineering towards a wide range of
further applications. Detailed structure/function analysis of these proteins is
important to facilitate their use in genetic engineering for various
applications.
Description:
Department of Biotechnology,
Cocbin University of Science and Technology
Sapna, K; Dr.Elyas, K K(Cochin University of Science And Technology, March , 2013)
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Abstract:
The microorganisms are recognized as important sources of protease
inhibitors which are valuable in the fields of medicine, agriculture and
biotechnology. The protease inhibitors of microbial origin are found to be versatile
in their structure and mode of inhibition that vary from those of other sources.
Although surplus of low molecular weight non-protein protease inhibitors from
microorganisms have been reported, there is a dearth of reports on proteinaceous
protease inhibitors. The search for new metabolites from marine organisms has
resulted in the isolation of more or less 10,000 metabolites (Fuesetani and
Fuesetani, 2000) many of which are gifted with pharmacodynamic properties. The
existence of marine microorganisms was reported earlier, and they were found to
be metabolically and physiologically dissimilar from terrestrial microorganisms.
Marine microorganisms have potential as important new sources of enzyme
inhibitors and consequently a detailed study of new marine microbial inhibitors
will provide the basis for future research (Imada, 2004).
Description:
Department of Biotechnology, Cochin University of Science and Technology
Manjula, R; Chandrasekaran, M(Cochin University of Science And Technology, May 19, 2014)
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Abstract:
The present study led to the recognition of Natrinema sp. BTSH 10 isolated
from saltern ponds, as an ideal candidate species for production of gelatinase, which
was noted as a halozyme capable of showing enzyme activity in the presence of 15%
NaCl. Results obtained during the course of the present study indicated potential for
application of this enzyme in industrial catalysis that are performed in the presence of
high concentrations of salt. The enzyme characteristics noted with this gelatinase also
indicate the scope for probable applications in leather industry, meat tenderization,
production of fish sauce and soy sauce. Since halophilic proteases are tolerant to
organic solvents, they could be used in antifouling coating preparations used to
prevent biofouling of submarine equipments. The gelatinase from haloarchaea could
be considered as a probable candidate for peptide synthesis. However, further studies
are warranted on this haloarcheal gelatinase particularly on structure elucidation and enzyme engineering to suit a wide range of applications. There is immense scope for
developing this halozyme as an industrial enzyme once thorough biochemistry of this
gelatinase is studied and a pilot scale study is conducted towards industrial production
of this enzyme under fermentation is facilitated. Based on the present study it is
concluded that haloarchaea Natrinema sp. that inhabit solar saltern ponds are ideal
source for deriving industrially important halozymes and molecular studies on
enzymes are prerequisite for their probable industrial applications. This is the first
time this species of archaea is recognized as a source of gelatinase enzyme that has
potential for industrial applications.
Divya, Jose; Dr.Mohandas, A; Dr.Bright Singh,I S(Cochin University of Science & Technology, November , 2011)
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Abstract:
In the present study,heterotrophic protease producing bacterial isolates were screened for protease activity and a potent protease producing bacterial isolate was selected,identified and coded as Pseudomonas aeruginosa MCCB 123.The organism was capable of producing three different types of enzymes each having potential industrial applications.The non-toxic nature of the bacterial strain and the relatively non-toxic nature of three enzymes suggested their poetential application in various industries.Application of LasA protease and beta-1,3 glucanase in DNA extraction is a promising area for commercial utilization. LasB protease can find its potential application in detergent and tanning industries.As on today Bacillus sp.has been the source of commercial proteases,and the ones produced form P.aeruginosa 123 can pave way for making the industrial and biomedical processes more cost effective and refined.
Description:
National Centre for Aquatic Health,Cochin University of Science and Technology