http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/469 Tue, 21 May 2013 21:03:35 GMT 2013-05-21T21:03:35Z http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2785 Molecular characterization of a crustin-like, putative antimicrobial peptide, Fi-crustin, from the Indian white shrimp, Fenneropenaeus indicus. Swapna P, Antony; Bright Singh, I S; Rosamma, Philip Fri, 01 Jan 2010 00:00:00 GMT http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2785 2010-01-01T00:00:00Z http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2784 Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV Swapna P, Antony; Bright Singh, I S; Sudheer, N S; Vrinda, S; Priyaja, P; Rosamma, Philip A crustinlike antimicrobial peptide from the haemocytes of giant tiger shrimp, Penaeus monodon was partially characterized at the molecular level and phylogenetic analysis was performed. The partial coding sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic of the shrimp crustins. Phylogenetic tree and sequence comparison clearly confirmed divergence of this crustinlike AMP from other shrimp crustins. The differential expression of the crustinlike AMP in P. monodon in response to the administration of various immunostimulants viz., two marine yeasts (Candida haemulonii S27 and Candida sake S165) and two bglucan isolates (extracted from C. haemulonii S27 and C. sake S165) were noted during the study. Responses to the application of two grampositive probiotic bacteria (Bacillus MCCB101 and Micrococcus MCCB104) were also observed. The immune profile was recorded preand postchallenge white spot syndrome virus (WSSV) by semiquantitative RTPCR. Expressions of seven WSSV genes were also observed for studying the intensity of viral infection in the experimental animals. The crustinlike AMP was found to be constitutively expressed in the animal and a significant downregulation could be noted postchallenge WSSV. Remarkable downregulation of the gene was observed in the immunostimulant fed animals prechallenge followed by a significant upregulation postchallenge WSSV. Tissuewise expression of crustinlike AMP on administration of C. haemulonii and Bacillus showed maximum transcripts in gill and intestine. The marine yeast, C. haemulonii and the probiotic bacteria, Bacillus were found to enhance the production of crustinlike AMP and confer significant protection to P. monodon against WSSV infection. Sat, 01 Jan 2011 00:00:00 GMT http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2784 2011-01-01T00:00:00Z http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2037 Antiviral property of marine actinomycetes against White Spot Syndrome Virus in penaeid shrimps Rosamma, Philip; Kumar, S; Achuthankutty, C T Aquaculture farms, particularly in Southeast Asia are facing severe crisis due to increasing incidences of White Spot Syndrome Virus (WSSV). Actinomycetes have provided many important bioactive compounds of high prophylactic and therapeutic value and are continually being screened for new compounds. In this communication, the results of a study made to determine the effectiveness of marine actinomycetes against the white spot disease in penaeid shrimps are presented. Twenty-five isolates of actinomycetes were tested for their ability to reduce infection due to WSSV among cultured shrimps. When these actinomycetes were made available as feed additives to the post-larvae of the black tiger shrimp Penaeus monodon for two weeks and challenged with WSSV, the post challenge survival showed variations from 11 to 83%. However, six isolates have shown to be the most potential candidates for further study. Sun, 01 Jan 2006 00:00:00 GMT http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2037 2006-01-01T00:00:00Z http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2036 Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Micrococcus MCCB 104 Using Central Composite Design Rosamma, Philip; Preetha, R; Jayaprakash, N S; Bright Singh, I S A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to VIbrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L). Mon, 01 Jan 2007 00:00:00 GMT http://dyuthi.cusat.ac.in:80/xmlui/handle/purl/2036 2007-01-01T00:00:00Z