Dr.Padma Nambisan
https://dyuthi.cusat.ac.in:443/xmlui/handle/purl/729
2024-02-07T16:36:27ZProline Accumulation and Salt Tolerance in Rice
https://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4736
Proline Accumulation and Salt Tolerance in Rice
Padma, Nambisan; Renuka, Devi P S; Sabu, A; Sheeja, T E
The morphological and biochemical response of calli and seedlings of different
rice cultivars were compared under acid saline conditions. Calli of
both tolerant and sensitive varieties showed severe stress symptoms like
browning and necrosis, but the onset of stress symptoms was delayed in
Pokkali. Seedlings of Pokkali showed minimal stress symptoms in lower
salinities, and curling and senescence of older leaves in higher salinities
although plants revived on amelioration of stress. Seedlings of the other
varieties showed severe stress symptoms even at low salinities and plant
death at higher salinities. Salt stress induced accumulation of the putative
osmoprotectant proline in calli and seedlings of all varieties. Proline accumulation
was higher in sensitive varieties than in Pokkali. These results
indicate that proline accumulation is not directly correlated with salt
tolerance in rice.
2013-01-01T00:00:00ZOptimization of Lignin Peroxidase, Manganese Peroxidase, and Lac Production from Ganoderma lucidum Under Solid State Fermentation of Pineapple Leaf
https://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4735
Optimization of Lignin Peroxidase, Manganese Peroxidase, and Lac Production from Ganoderma lucidum Under Solid State Fermentation of Pineapple Leaf
Padma, Nambisan; Sudha, Hariharan
This study was undertaken to isolate ligninase-producing white-rot fungi for use in the extraction of fibre from pineapple leaf agriwaste. Fifteen fungal strains were isolated from dead tree trunks and leaf litter. Ligninolytic enzymes (lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac)), were produced by solid-state fermentation (SSF) using pineapple leaves as the substrate. Of the isolated strains, the one showing maximum production of ligninolytic enzymes was identified to be Ganoderma lucidum by 18S ribotyping. Single parameter optimization and response surface methodology of different process variables were carried out for enzyme production. Incubation period, agitation, and Tween-80 were identified to be the most significant variables through Plackett-Burman design. These variables were further optimized by Box-Behnken design. The overall maximum yield of ligninolytic enzymes was achieved by experimental analysis under these optimal conditions. Quantitative lignin analysis of pineapple leaves by Klason lignin method showed significant degradation of lignin by Ganoderma lucidum under SSF
BioResources 8(1), 250-271.
2013-01-01T00:00:00ZEvaluation of Somaclonal Variation in Callus Cultures of Jatropha curcas Maintained on Different Hormonal Combinations Using RAPD Markers
https://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4734
Evaluation of Somaclonal Variation in Callus Cultures of Jatropha curcas Maintained on Different Hormonal Combinations Using RAPD Markers
Padma, Nambisan; Jikku, Jose; Nimisha, K; Anu, M A
Random genetic changes generated during in vitro culture are not desirable for plant
micropropagation and genetic transformation. RAPD markers were used to detect the variation in leaf disc callus
cultures of Jatropha curcas, maintained in Murashige and Skoog (MS) medium with different auxin and
cytokinin combinations. In total 41 scorable bands were produced with 11 primers. Out of 41 bands, 37 were
polymorphic (91.12%). The average number of polymorphic bands was 3.36 per primer. The highest similarity
(0.82) with mother plant was seen in callus maintained on MS with hormonal combination Indole butyric
acid - 0.4mg/l+ N6-benzyladenine purine - 4.0 mg/l. The callus grown on MS with hormonal combinations
IBA- 0.4mg/l+ BAP- 2.0mg/l, IBA- 0.4mg/l+ BAP- 2.5mg/l and IBA- 0.6 mg/l+ BAP- 2.0 mg/l also showed
similarity with the mother plant. Callus maintained on MS with hormonal combination IBA- 0.2mg/l+ BAP- 2.0
mg/l was found to show least similarity (0.53) with mother plant
World Journal of Agricultural Sciences 8 (6): 616-623, 2012
2012-01-01T00:00:00ZBiodegradation Of Phenol Using Spent Substrate Of Pleurotus Sp.
https://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4733
Biodegradation Of Phenol Using Spent Substrate Of Pleurotus Sp.
Padma, Nambisan; Jasmin, Koshy; Neethu, Chandran
Phenol is an aromatic hydrocarbon which exists as a colorless or
white solid in its pure state. Over the past several decades, there is
growing concern about wide spread contamination of surface and
ground water by phenol, due to rapid development of chemical and
petrochemical industries. Phenol affects aquatic life even at relatively
low concentration (5-25mg/L). Treatment for removal of phenol
includes chemical as well as biological processes. Studies show that
ligninases such as Lignin Peroxidase and Laccase, produced by
Pleurotus sp., can degrade phenol. Spent substrate of Pleurotus
mushrooms consists of ligninases. Present work was to investigate the
potential of spent substrate of edible mushroom P. ostreatus for
biodegradation of phenol. P. ostreatus was cultivated on paddy straw.
After harvest, spent substrate was utilized for phenol degradation.
According to the enzyme profile of two ligninases present in the spent
substrate of P. ostreatus, maximum specific activity for Laccase was observed in 35 day old
spent substrate and LiP activity was maximum in 56 day old spent substrate, which together
contributed significantly for removal of phenol. Spent substrate of 35th and 56th day were
each incubated with phenol sample (1:1w/v) for one day, which resulted in degradation of
phenol by 48% and 45% respectively. From these results it appears that, spent substrate of P.
ostreatus can be used effectively to remove phenol from industrial effluents
World Journal Of Pharmacy And Pharmaceutical Sciences,Volume 1, Issue 2, 656-661.
2012-07-10T00:00:00Z