Dr. M Chandrasekharanhttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/7312024-02-07T07:40:51Z2024-02-07T07:40:51ZMarine Bacteria As Source Of Pigment For Application As Dye In Textile IndustryChandrasekaran, MJissa, Krishna GSoorej, Basheer MBeena, P Shttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/42702014-07-23T20:30:39Z2008-01-01T00:00:00ZMarine Bacteria As Source Of Pigment For Application As Dye In Textile Industry
Chandrasekaran, M; Jissa, Krishna G; Soorej, Basheer M; Beena, P S
The textile industry is one amongst the rapidly growing industries world wide, which utilizes enormous
amounts of synthetic dyes. Consequently, the effluent from these textile industries poses serious threat to the
environment which is often very difficult to treat and dispose. This has become a very grave problem in environment
conservation and hence natural pigments have drawn the attention of industry as safe alternative. In this context,
in the present study an attempt was made to bioprospect marine bacteria towards isolation of a suitable and ideal
pigment that could be used as a natural dye. A marine Serratia sp. BTWJ8 was recognized to synthesize enormous
amounts of a prodigiosin-like pigment. The pigment was isolated and characterized for various properties. The
pigment was evaluated for application as a dye in the textile industry. Results of the studies indicated that this
pigment could be used as a natural dye for imparting red-yellow colour to various grades of textile materials. The
colour was observed to be stable after wash performance studies
Proc. Internatl. Conf. Biodiv. Conserv. & Mgt., 2008 : 743 - 4.
2008-01-01T00:00:00ZBiocompatible polyhydroxybutyrate (PHB) production by marine Vibrio azureus BTKB33 under submerged fermentationChandrasekaran, MRaghul, Subin SasidharanSarita,G Bhathttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/42692014-07-23T20:30:39Z2014-03-17T00:00:00ZBiocompatible polyhydroxybutyrate (PHB) production by marine Vibrio azureus BTKB33 under submerged fermentation
Chandrasekaran, M; Raghul, Subin Sasidharan; Sarita,G Bhat
Polyhydroxybutyrate (PHB) is known to have applications
as medical implants and drug delivery carriers and
is consequently in high demand. In the present study the
possibilities of harnessing potential PHB-producing vibrios
from marine sediments as a new source of PHB was investigated
since marine environments are underexplored. Screening
of polyhydroxyalkanoate (PHA)-producing vibrios from
marine sediments was performed using a fluorescent plate
assay followed by spectrophotometric analysis of liquid cultures.
Out of 828 isolates, Vibrio sp. BTKB33 showed maximum
PHA production of 0.21 g/L and PHA content of
193.33 mg/g of CDW. The strain was identified as Vibrio
azureus based on phenotypic characterization and partial
16S rDNA sequence analysis. The strain also produced several
industrial enzymes: amylase, caseinase, lipase, gelatinase,
and DNase. The FTIR analysis of extracted PHA and its
comparison with standard PHB indicated that the accumulated
PHA is PHB. Bioprocess development studies for enhancing
PHA production were carried out under submerged fermentation
conditions. Optimal submerged fermentation conditions
for enhanced intracellular accumulation of PHA production
were found to be 35 °C, pH −7, 1.5 % NaCl concentration,
agitation at 120 rpm, 12 h of inoculum age, 2.5 % initial
inoculum concentration, and 36 h incubation along with supplementation
of magnesium sulphate, glucose, and ammonium
chloride. The PHA production after optimization was
found to be increased to 0.48 g/L and PHA content to426.88 mg/g of CDW, indicating a 2.28-fold increase in
production. Results indicated that V. azureus BTKB33 has
potential for industrial production of PHB.
Ann Microbiol
DOI 10.1007/s13213-014-0878-z
2014-03-17T00:00:00ZTrypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial OriginChandrasekaran, MManzur Ali, P PRekha Mol, K RSapna, KSarita,G Bhathttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/42682014-07-23T20:30:40Z2014-03-11T00:00:00ZTrypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin
Chandrasekaran, M; Manzur Ali, P P; Rekha Mol, K R; Sapna, K; Sarita,G Bhat
Protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and
food preservative applications. Fungi have been recognized as sources of protease inhibitors,
although there are only few such reports on mushrooms. This work reports the purification and
characterization of a trypsin inhibitor from the fruiting body of edible mushroom Pleurotus
floridanus (PfTI) and its effect on the activity of microbial proteases. The protease inhibitor was
purified up to 35-fold by DEAE-Sepharose ion exchange column, trypsin-Sepharose column and
Sephadex G100 column. The isoelectric point of the inhibitor was 4.4, and its molecular mass was
calculated as 37 kDa by SDS-PAGE and 38.3 kDa by MALDI-TOF. Inhibitory activity confirmation
was by dot-blot analysis and zymographic activity staining. The specificity of the inhibitor
toward trypsin was with Ki of 1.043×10−10 M. The inhibitor was thermostable up to 90 °C with
maximal stability at 30 °C, active over a pH range of 4–10 against proteases from Aspergillus
oryzae, Bacillus licheniformis, Bacillus sp. and Bacillus amyloliquefaciens. Results indicate the
possibility of utilization of protease inhibitor from P. floridanus against serine proteases
Appl Biochem Biotechnol (2014) 173:167–178
DOI 10.1007/s12010-014-0826-1
2014-03-11T00:00:00ZArtificial neural network modeling for surface roughness prediction in cylindrical grinding of Al‐SiCp metal matrix composites and ANOVA analysisChandrasekaran, MDevarasiddappa, Dhttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/42672014-07-23T20:30:39Z2014-06-01T00:00:00ZArtificial neural network modeling for surface roughness prediction in cylindrical grinding of Al‐SiCp metal matrix composites and ANOVA analysis
Chandrasekaran, M; Devarasiddappa, D
Metal matrix composites (MMC) having aluminium (Al) in the matrix phase and silicon
carbide particles (SiCp) in reinforcement phase, ie Al‐SiCp type MMC, have gained
popularity in the re‐cent past. In this competitive age, manufacturing industries strive to
produce superior quality products at reasonable price. This is possible by achieving higher
productivity while performing machining at optimum combinations of process variables. The
low weight and high strength MMC are found suitable for variety of components
Advances in production engineering and management,vol 9 no 2,pp 59-70
2014-06-01T00:00:00Z