Seema,P V; Sudha,B; Pius S,Padayatti; Asha,Abraham; Raghu,K G; Paulose,C S(Department of Biotecnology, June , 1996)
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Abstract:
The functional basis of diabetes-mellitus to a certain extent,
can be elucidated by studying diabetes-induced changes
in metabolic enzymes. Malate dehydrogenase (MDH),
is an enzyme directly involved in glucose metabolism. The
kinetic parameters of MDH and its purified cytosolic isozyme,
S-MDH, have been studied in the liver of streptozotocin-
diabetic rats; also the potential of the leaf extract of A.
marmelose as an was investigated. The
Km of the liver enzyme increased significantly, in both
crude and purified preparations in the diabetic state when
compared to Lhe respective controls. Insulin as well as leaf-
•extract treatment of the diabetic rats brought about a reversal
of K. values to near normal. Vmax of purified S-MDH
was significantly higher in the diabetic state when compared
to the control. Insulin and leaf extract treatment did not
reverse this change. Since MDH is an important enzyme in
glucose metabolism, the variation in its quantitative and
qualitative nature may contribute to the pathological status
of diabetes. The fact that leaf extract of A. marmelose
was found to be as effective as insulin in restoration of blood
glucose and body weight to normal levels, the use of A.
marmelose as potential hypoglycemic agent is suggested.
Harilal, S S; Riju C, Issac; Bindhu, C V; Geetha K, Varier; V P N Nampoori; Vallabhan, C P G(Pramana, February , 1996)
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Abstract:
Analysis of the emission bands of the CN molecules in the plasma generated from
a graphite target irradiated with 1-06/~m radiation pulses from a Q-switched Nd:YAG laser has
been done. Depending on the position of the sampled volume of the plasma plume, the intensity
distribution in the emission spectra is found to change drastically. The vibrational temperature
and population distribution in the different vibrational levels have been studied as function of
distance from the target for different time delays with respect to the incidence of the laser pulse.
The translational temperature calculated from time of flight is found to be higher than the
observed vibrational temperature for CN molecules and the reason for this is explained.
Waliaula Mola,P; Sudha,B; Paulose,C S(Department of Biotechnolgy, November , 1996)
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Abstract:
The effect of insulin on cell proliferation in vivo has been studied in hepatectomised
streptozotocin- diabetic rats. The extent of cell proliferation in sham and hepatectomized- control,
diabetic and insulin treated rats were monitored by determining DNA content and [3H]thymidine
incorporation into DNA. The kinetic parameters of thymidine kinase a regulatory enzyme for
DNA synthesis was also studied in these groups. The rate of DNA synthesis in liver of
streptozotocin -diabetic rats was significantly higher 24 hrs post-hepatectomy compared to
control and insulin treated diabetic groups. Kinetic studies of thymidine kinase revealed that there
was no change in the Michaelis -Menten constant (Km) whereas maximum velocity (Vmax) was
elevated in the diabetic hepatectomized groups compared to control and insulin treated
hepatectomized groups. Thus our study elucidates the role of insulin in thymidine kinase activity
and DNA synthesis.