Pius S,Padayatti; Paulose,C S(Department of Biotechnolgy, April 2, 1999)
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Abstract:
The brain stems (13S) of streptozotocin (STZ)-diabetic rats were studied lo see the changes in
neurotransmitter content and their receptor regulation. The norepinephrine (NE) content
determined in the diabetic brain stems did ^ control. an E showed la
while
PI turnover
hri
content increased significantly compared N^r eNveFa o the recep
significant increase. The alpha2 adrenergic receptor
IneP utisoulinntreat d ratsetheNE contentt dec^ sled
was significantly reduced during diabetes. in
versedcto reanorm
sed
ulcrea e tK
reatment
the
state.
while EPI content remained increased as in die diabetic
B,, for a]pha2 adrenergic receptors slw^nificantly while
Unlabelled clonidine inhibited [31-I]NE binding in BS of control, diabetic and insulin treated
ulations bindi
diabetic rats showed that alpha2 adrenergicre^ punks cojnidiabetic animal the ligand bound
sites with Hill slopes significantly away from unity.
weaker to the low affinity site than in controls. Insulin treatment reversed[ this allumbmn to
control levels. The displacement analysis using (-)-epinephrine age in
control and diabetic animals revealed two populations of receptor affinidtyo=tat ss. In control
animals, when GTP analogue added with epinephrine, the curve nagnlde caofnfitnroit yS
model; but in the diabetic BS this effect `not
aobserved. In
bintact
oth the diabetic
data thus showlthat
the effects of monovalent cations on affinity
alphaz adrenergic receptors have a reduced affinity v
due
in stem ialtered Itscppeomson(5-
regulation. The serotonin (5-HT) coat
hydroxy) tryptophan (5-HTP) showed an increase and its breakdown metabolite (5-hydroxy)
indoleacetic acid (5-I{IAA) showed a significant decrease. This showed that in serotonergic
which l
nerves there is a disturbance in both synthetic and breankduomwnbers pretma'med ana
increased 5-HT. The high affinity serotonin receptor um ese serotonerg
decrease in the receptor affinity. The insulin ^treatmentsturtiy showsha decreased serotonergic
receptor kinetic parameters to control level.
receptor function. These changes in adrenergic and serotonergic receptor function were
suggested to be important in insulin function during STZ diabetes.
Girish Kumar, K; Chowdary, K P R; Devala Rao, G(Indian Journal Of Pharmaceutical Sciences, January 8, 1999)
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Abstract:
Two simple and sensitive spectrophotometric methods (A and B)in the visible region have been developed for the determination of nimesulide in bulk and in dosage forms.Method A is based on the reaction of reduced nimesulide with nitrous acid followed by its coupling with phloroglucinol to yield an yellow colored azo dye with an absorption maximum of 400 nm and method B is based on the reaction of reduced nimesulide with p-dimethylamino benzaldehyde(PDAB) to form an yellow colored chromogen wiht an absorption maximum of 415 nm.When pharmaceutical preparations (Tablets and suspension) were analysed, the results obtained by the proposed methods are in good agreement with the labelled amounts and are comparable with the results obtained by a reported method.recovery in both the method is 98-101 %.
Sabu,A; Chandrasekaran,M(Cochin University of Science and Technology, December , 1999)
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Abstract:
This study presents the L-Glutaminase Production by Marine Fungi. Enzymes are involved in all aspects of biochemical conversion from the simple enzyme or fermentation conversion to the complex techniques in genetic engineering. Enzyme industry is one among the major industries of the world and there exists a great market for enzymes in general. Food industry is recognized as the largest consumer for commercial enzymes (Lon sane and Ramakrishna, 1989). In industry, enzymes are frequently used for process improvement, for instance to enable the utilization of new types of raw materials or for improving the physical properties of a material so that it can be more easily processed. They are the focal point of biotechnological processe. The marine biosphere is one of the richest of the earth's innumerable habitats, yet is one of the least well characterized. The marine biosphere covers more than two third of the world's surface, our knowledge of marine microorganisms, in particular fungi, is still very limited (Molitoris and Schumann, 1986). The results obtained in the present study the following conclusions are drawn. Beauveria bassiana isolated form marine sediment has immense potential as an Industrial organism for production of L-glutaminase as an extracellular enzyme employing either submerged fermentnation or solid state fermentation
Sugunan, S; Bindhu, Jacob(Indian Journal of Chemistry, October , 1999)
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Abstract:
The acidity of the various rare-earth exchanged zeolite-Y
catalysts has been examined by titration method using Hammett
indicators and is correlated with the catalytic activity of the
samples in the benzylation of 0-xylene.