Mohanan,Valiyaveettil; Finla, Chathu; Paulose, Cheramadathikudyil S(Department of Bio Technology, December 1, 2004)
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Abstract:
The purpose of this study was to investigate the role of central 5-HT2C receptor binding in rat model of pancreatic regeneration
using 60-70% pancreatectomy. The 5-HT and 5-HT2c receptor kinetics were studied in cerebral cortex and brain stem of sham
operated, 72 h pancreatectomised and 7 days pancreatectomised rats. Scatchard analysis with [3H] mesulergine in cerebral
cortex showed a significant decrease (p < 0.05) in maximal binding (B^,ax) without any change in Kd in 72 h pancreatectomised
rats compared with sham. The decreased Bmax reversed to sham level by 7 days after pancreatectomy. In brain stem , Scatchard
analysis showed a significant decrease (p < 0.01) in Bax with a significant increase (p < 0.01) in Kd. Competition analysis
in brain stem showed a shift in affinity towards a low affinity. These parameters were reversed to sham level by 7 days after
pancreatectomy. Thus the results suggest that 5-HT through the 5-HT2C receptor in the brain has a functional regulatory role in
the pancreatic regeneration. (Mol Cell Biochem 272: 165-170, 2005)