Bright Singh, I S; Rosamma, Philip; Anas, A; Somnath Pai, S; Douglas, W Lowman; David, L Williams; Stewart, Millen(Blackwell Publishing, July 1, 2009)
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Abstract:
E¡ect of an extraction method on the structure of
glucan and its immunostimulatory response in Fenneropenaeus
indicus was investigated. Here we extracted
alkali insoluble glucan (AIG) and alkali
soluble glucan (ASG) from a ¢lamentous fungi Acremonium
diospyri following alkali^acid hydrolysis and
the sodium hypochlorite oxidation and dimethyl
sulphoxide extraction method respectively. Structural
analysis showed that 85% of glucan in AIG was a
(1 !3)-b-D-glucan and it increased the prophenoloxidase
and reactive oxygen intermediate activity
when administered to F. indicus. On the other hand,
ASG, which contained 93% (1 !3)-a-glucan, did
not induce signi¢cant immune response in shrimp.
Here we report that the di¡erence in immunostimulatory
potential between AIG and ASG is due to the
di¡erence in the percentage of (1 !3)-b-D-glucans
present in each preparation, which varies with the
method of extraction employed. Also our observations
suggest that glucan can be used as a potential
immunostimulant to shrimp, provided it contains
(1 !3)-b-D-glucan as the major fraction.
Sarlin,P J; Dr.Rosamma,Philip(Cochin University of Science & Technology, 2005)
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Abstract:
The study revealed the potential of marine yeasts as a source of
single cell protein and immunostimulant for prawns. Prawns fed with
the selected marine yeasts were showing more growth compared to the
control feed and commercial feed. Yeasts being rich with proteins,
vitamins and carbohydrates serve as a growth promoter for prawns as
being evidenced in this study. The better performance of marine
yeasts, D. hansenii S8 and S100 and C. tropicalis S186 compared to S.
cerevisiae S36 as a feed supplement is worth investigating. Besides
being a rich nutritional source, yeasts act as immunostimulants by
virtue of its high carbohydrate (Beta, 1-3 glucan) and RNA content. Beta, 1-3
glucan, a cell wall component of yeasts /fungi is the most commonly
used immunostimulant in aquaculture. The present study shows that
even the whole cell yeast could serve as a good immunostimulant
when supplied through diet. Extraction of Beta-1,3 glucan results in the
removal of nutrients like proteins, vitamins etc. from the cell biomass.Utilization of the yeast biomass as such in the diet would help perform
a dual role as nutritional component and immunostimulant for
aquaculture applications.
Description:
Dept.of Marine Biology, Microbiology and Biochemistry,
Cochin University of Science and Technology