Chandrasekaran, M; Rajeev Kumar, S; Sabu, A; Suresh, P V; Keerthi, T R(Kluwer Academic Publishers, August 6, 1999)
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Abstract:
Beauveria sp. BTMF S10 isolated from marine sediment produced extracellular L-glutaminase. Maximal L-
glutaminase yield (46.9 U/ml) was obtained in a medium supplemented with 1% (w/v) yeast extract and sorbitol,
9% (w/v) sodium chloride and 0.2% (w/v) methionine, initial pH 9.0 and at 27 °C after 108 h. This enzyme was
inducible and growth-associated.
Description:
World Journal of Microbiology & Biotechnology 15: 751±752, 1999.
Chandrasekaran, M; Nagendra, Prabhu G(Elsevier, 1997)
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Abstract:
Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation
(SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry
substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium
dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while
nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg
protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system
Description:
Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997
Sabu, A; Chandrasekaran, M(Cochin University of Science and Technology, December , 1999)
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Abstract:
This study presents the L-Glutaminase Production by Marine Fungi. Enzymes are involved in all aspects of biochemical conversion from the simple enzyme or fermentation conversion to the complex techniques in genetic engineering. Enzyme industry is one among the major industries of the world and there exists a great market for enzymes in general. Food industry is recognized as the largest consumer for commercial enzymes (Lon sane and Ramakrishna, 1989). In industry, enzymes are frequently used for process improvement, for instance to enable the utilization of new types of raw materials or for improving the physical properties of a material so that it can be more easily processed. They are the focal point of biotechnological processe. The marine biosphere is one of the richest of the earth's innumerable habitats, yet is one of the least well characterized. The marine biosphere covers more than two third of the world's surface, our knowledge of marine microorganisms, in particular fungi, is still very limited (Molitoris and Schumann, 1986). The results obtained in the present study the following conclusions are drawn. Beauveria bassiana isolated form marine sediment has immense potential as an Industrial organism for production of L-glutaminase as an extracellular enzyme employing either submerged fermentnation or solid state fermentation