Renu, S; Dr.Chandrasekaran,M(Cochin University of Science and Technology, October , 1991)
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Abstract:
L-glutaminases (L—glutamine amidohydrolase EC.3.5.l.2) is proposed as a prospective candidate for enzyme therapy cnf cancer and also as zui important additive during enzymatic digestion of shoyu koji since it could enhance glutamate content of soysauce. Commercial production of glutaminase could make possible its wide application in these areas, which would demand availability of potential sources and suitable fermentation techniques. The ‘present investigation highlighted marine environment as a potential source of efficient glutaminase producing bacteria mainly species of pseudomonas, aeromonas ,vibrio,alcaligenes, acinetobacter bacillus and planococci.Among them pseudomonas fluorescens ACMR 267 and v.cholerae ACMR 347 were chosen as the ideal strains for glutaminase production.Extracellular glutaminase fraction from all strains were in higher titres than intracellular enzymes during growth in mineral media, nutrient broth and nutrient broth added with glutamine.Glutaminase from all strains were purified employing (NH4)2SO4 fractionation followed tnr dialysis and ion exchange chromatography. The purified glutaminase from all strains were observed to be active and stable over a wide range of gfii and temperature.Optimization studies cflf environmental variables that normally influence time yiehi of glutaminase indicated that the optimal requirements of these bacteria for maximal glutaminase production remained stable irrespective of the medium, they are provided with for enzyme production. However, solid state fermentation technique was observed to
be the most suitable process for the production of Glutaminase.
Description:
Department of Applied Chemistry, Cochin
University of Science and Technology
Chandrasekaran, M; Rajeev Kumar, S; Keerthi, T R; Sabu, A(Elsevier, September 27, 1999)
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Abstract:
Extracellular L-glutaminase production by Beau6eria sp., isolated from marine sediment, was observed during solid state
fermentation using polystyrene as an inert support. Maximal enzyme production (49.89 U:ml) occurred at pH 9.0, 27°C, in a
seawater based medium supplemented with L-glutamine (0.25% w:v) as substrate and D-glucose (0.5% w:v) as additional carbon
source, after 96 h of incubation. Enzyme production was growth associated. Results indicate scope for production of salt tolerant
L-glutaminase using this marine fungus
Chandrasekaran, M; Nagendra, Prabhu G(Rapid Science Publishers, 1995)
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Abstract:
Polystyrene beads, impregnated with mineral salts/glutamine medium as inert support, were used to produce
L-glutaminase from Vibrio costicola by solid-state fermentation. Maximum enzyme yield, 88 U/g substrate, was
after 36 h. Glucose at 10 g/kg enhanced the enzyme yield by 66%. The support system allowed glutaminase to
be recovered with higher specific activity and lower viscosity than when a wheat-bran system was used
Description:
World Journal of Microbiology & Biotechnology 11,683-684