Anas, A; Bright Singh, I S(Centre for Fish Disease Diagnosis and Management, School of Environmental Studies, 2005)
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Abstract:
Present work is aimed at development of an appropriate microbial technology for protection of larvae of macrobrachium rosenbergii from disease and to increase survival rate in hatcheries. Application of immunostimulants to activate the immune system of cultured animals against pathogen is the widely accepted alternative to antibiotics in aquaculture. The most important immunostimulant is glucan. Therefore a research programme entitled as extraction of glucan from Acremonium diospyri and its application in macrobrachium rosenbergii larval rearing system along with bacterians as microspheres. The main objectives of the study are development of aquaculture grade glucan from acremonium diospyri, microencapsulated drug delivery system for the larvae of M. rosenbergii and microencapsulated glucan with bacterian preparation for the enhanced production of M. rosenbergii in larval rearing system. Based on the results of field trials microencapsulated glucan with bacterin preparation, it is concluded that the microencapsulated preparation at a concentration of 25g per million larvae once in seven days will enhance the production and quality seed of M. rosenbergii.
Bright Singh, I S; Sajeevan, T P; Rosamma, Philip(Elsevier, March 5, 2006)
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Abstract:
The efficacy of a marine yeast Candida sake as source of immunostimulant to Indian white shrimp Fenneropenaeus indicus was
estimated. Biomass of C. sake was prepared using malt extract agar and incorporated at graded levels into a standard diet to prepare
yeast diets of varying biomass concentrations (1%, 10% and 20%). F. indicus were fed on these diets for a period of 28 days and
challenged orally with white spot syndrome virus (WSSV) and immune parameters such as total haemocyte count, phenoloxidase
and nitroblue tetrazolium reduction (NBT) were determined. Ten per cent C. sake in the diet was found to support an optimum
immune response in the animals in general and their enhancement could be observed on the second and third day following
challenge with the virus. The study has demonstrated that marine yeast C. sake at 10% in diet (w/w) may be used as an effective
source of immunostimulants in F. indicus