Finla, Chathu; Dr. Paulose, C S(Cochin University of Science and Technology, February , 2007)
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Abstract:
In the present work, the role of oxygen, epinephrine and glucose
supplementation in regulating neurotransmitter contents, adrenergic and glutamate
receptor binding parameters in the cerebral cortex of experimental groups of neonatal
rats were investigated. The study of neurotransmitters and their receptors in the
cerebral cortex and the EEG pattern in the brain regions of neonatal rats were taken as
index for brain damage due to hypoxia, oxygen and epinephrine. Real-Time PCR work was done to confirm the binding parameters. Second messenger, cyclic
Adenosine Monophosphate (cAMP) was assayed to find the functional correlation of
the receptors. Behavioural studies were carried out to confirm the biochemical and
molecular studies. The efficient and timely supplementation of glucose plays a crucial
role in correcting the molecular changes due to hypoxia, oxygen and epinephrine. The
addictive neuronal damage effect due to oxygen and epinephrine treatment is another
important observation. The corrective measures from the molecular study brought to
practice will lead to maintain healthy intellectual capacity during the later
developmental stages, which has immense clinical significance in neonatal care.
Description:
Department of Biotechnology, Cochin
University of Science and Technology
Paulose,C S; Athira, Babu; Anju, T R(Indian Journal of Biochemistry and Biophysics, April , 2009)
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Abstract:
Hypoxia is one of the major causes of damage to the fetal and neonatal brain and cardiac functions. in earlier studies we have reported the brain damage caused by hypoxia and resusciation with oxygen and epinephrine and have found that glucose treatment to hypoxic rats and hypoxic rats treated with oxygen shows a reversal of brain damage. during this study the findings may have clinical significance in the proper management of heart and brain functions.