Madhu, K M; Dr.Chandrasekaran,M(Cochin University of Science and Technology, April , 2007)
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Abstract:
The beta-glucosidase enzyme purified from the marine fungus, Aspergillus
sydowii BTMFS 55 showed a good yield of enzyme production under solid state
fermentation. The statistical optimization of the media components revealed that
moisture content, concentration of peptone and inoculum are the major parameters
which supported the maximal enzyme production. The purified enzyme showed low
pH activity and stability, glucose tolerance and activation by ethanol. It could produce
ethanol from wheat bran and rice straw by simultaneous saccharification and
fermentation with yeast.The glucosidase purified from Aspergillus sydowii BTMFS 55
shows great potential for several biotechnological applications such as the production
of bio-ethanol from agricultural biomass and improvement in the aromatic character
of wines and fruit juices through the hydrolysis of flavour glucosidic precursors.
There is immense scope for the application of this marine fungus in the biofuel
production besides in other industries provided further studies are pursued in
exploiting this enzyme and the organism particularly scale up studies with respect to
application. There is also ample scope for cloning of the gene encoding beta-glucosidase
in domesticated hosts such as Pichia pastoris or S. cerevisiae that can produce ethanol
directly from cellulosic biomass.
Description:
Department of Biotechnology,
Cochin University of Science and Technology
Nagendra, Prabhu G; Dr.Chandrasekaran,M(Cochin University of Science and Technology, June , 1996)
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Abstract:
Use of inert supports have been recommended for SSF in on ar to
overcome its inherent problems and efforts are being made to search
for newer and better materials to act as inert solid supports lidoo
et al, 1982; Zhu et al, 1994).In the present study an attempt is made to produce L-glutaminase, which is industrially and therapeutically impo rtant, from marine bacteria under solid state fermentation using natura.l.
inert and mixed substrates with a view to develop an ideal bioprocess
for its large scale production.
Description:
Microbial Technology Unit, Department of Biotechnology, Cochin University of Science and Technology
Chandrasekaran, M; Sreeja, Chellappan; Jasmin, C; Soorej, Basheer M; Elyas, K K; Sarita,G Bhat(Elsevier, October 17, 2005)
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Abstract:
Engyodontium album isolated from marine sediment produced protease, which was active at pH 11. Process parameters influencing the
production of alkaline protease by marine E. album was optimized. Particle size of <425 mm, 60% initial moisture content and incubation at 25 8C
for 120 h were optimal for protease production under solid state fermentation (SSF) using wheat bran. The organism has two optimal pH (5 and 10)
for maximal enzyme production. Sucrose as carbon source, ammonium hydrogen carbonate as additional inorganic nitrogen source and amino acid
leucine enhanced enzyme production during SSF. The protease was purified and partially characterized. A 16-fold purified enzyme was obtained
after ammonium sulphate precipitation and ion-exchange chromatography. Molecular weight of the purified enzyme protein was recorded
approximately 38 kDa by SDS-PAGE. The enzyme showed maximum activity at pH 11 and 60 8C. Activity at high temperature and high alkaline
pH suggests suitability of the enzyme for its application in detergent industry