Bright Singh, I S; Seena, Jose; Rosamma, Philip; Mohandas, A; Jayesh, P(Elsevier, December 26, 2010)
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Abstract:
Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and
its export value. In this context a primary haemocyte culture developed from Penaeus monodon was
employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium
chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos.
Using MTT assay 12 h IC50 values calculated were 31.09 16.27 mM and 5.52 1.16 mM for cadmium
chloride and mercuric chloride and 59.94 52.30 mg l 1 and 186.76 77.00 mg l 1 for malathion and
monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on
haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study
suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of
aquaculture drugs, management chemicals and pollutants