Hatha, A A M; George, M; Anjumol, A; George, G(Academic Journals, March 16, 2012)
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Abstract:
Emergence of drug resistance among pathogenic bacteria to currently available antibiotics has intensified the search for novel bioactive compounds from unexplored habitats. In the present study actinomycetes were isolated from two relatively unexplored and widely differing habitats such as mountain and wetlands and their ability to produce antibacterial substances were analyzed. Pure cultures of actinomycetes were identified by morphological and biochemical tests. Various genera of actinomycetes encountered included Nocardia, Pseudonocardia, Streptomyces, Nocardiopsis, Streptosporangium, Micromonospora, Rhodococcus, Actinosynnema, Nocardiodes, Kitasatosporia, Gordona, Intrasporangium and Streptoalloteichus. The frequency of occurrence of each genus was found to vary with sample. About 47% of wetland isolates and 33% of mountain isolates were identified as various species of Nocardia. The isolated strains differed among themselves in their ability to decompose proteins and amino acids and also in enzyme production potential. Antibiotic activities of these actinomycetes were evaluated against 12 test pathogenic bacteria by well diffusion method using agar wells in glycerol-yeast extract agar. About 95% of actinomycete isolates from wetland ecosystem and 75% of highland isolates suppressed in different degrees the growth of test pathogens. Relatively high antibacterial activity among these isolates underlined their potential as a source of novel antibiotics.
Description:
African Journal of Microbiology Research Vol. 6(10), pp. 2265-2271, 16 March, 2012
Hatha, A A M; Maya, George; Neethu, Cyriac; Aswathi, Nair(Indian Journal Of Geo Marine Sciences, June , 2011)
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Abstract:
Diversity of different groups of Bacillus and Actinomycetes in the water and sediment samples from kumarakom estuary was analysed to find out potential strains for further application. Bacillus genera was identified and grouped into five phenogroups .Phenogroups show differences in the shape of the spore,position of the spore,and swelling of the sporangium.Ability of the isolates to elaborate various hydrolytic enzymes and their ability to reduce nitrate and ferment various carbohydrate sources were also studied.
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Indian Journal Of Geo Marine Sciences Vol.40(3),June 2011,PP 430-437
Dhanya,P R; Dr.Saleena, Mathew(Cochin University of Science And Technology, October 16, 2012)
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Abstract:
Unprocessed seafood harbor high number of bacteria, hence are more prone to
spoilage. In this circumstance, the use of spice in fish for reduction of microorganism can
play an important role in seafood processing. Many essential oils from herbs and spices are
used widely in the food, health and personal care industries and are classified as GRAS
(Generally regarded as safe) substances or are permitted food additives. A large number of
these compounds have been the subject of extensive toxicological scrutiny. However, their
principal function is to impart desirable flavours and aromas and not necessarily to act as
antimicrobial agents. Given the high flavour and aroma impact to plant essential oils, the
future for using these compound as food preservatives lies in the careful selection and
evaluation of their efficacy at low concentrations but in combination with other chemical
preservatives or preservation processes. For this reason they are worth of study alone or in
combination with processing methods in order to establish if they could extend the shelf-life
of foods.
In this study, the effect of the spices, clove, turmeric, cardamom, oregano, rosemary
and garlic in controlling the spoilage and pathogenic bacteria is investigated. Their effect on
biogenic amine formation in tuna especially, histamine, as a result of bacterial control is also
studied in detail. The contribution of spice oleoresin in the sensory and textural parameters
is investigated using textural profile analysis and sensory panel. Finally, the potential of
spices in quality stabilization and in increasing the shelf–life of tuna during frozen storage is
analysed
Description:
School of Industrial Fisheries
Cochin University of Science and Technology
Hatha, A A M; Thirupathi, Suresh; Doriaraj, Sreenivasan; Sangeetha, Sreenivasan; Lakshmanaperumalsamy, P(Microbes And Enviornments, October 13, 2004)
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Abstract:
The source of samonella cross contamination in 15 retail chicken outlets in aresidual area in coimbatore city ,sourthern India was studied. Chopping boards and the butchers hands were predominant followed by knives and the weighing balance tray. Serotyping of the salmonella strains revealed that all strains were salmonella enteritis, except one which was found to be salmonella cerro.The anti bacterial activity of commonly used spices were evaluated.
Hatha, A A M; Nishamol, S; Suchitra, R; Rinoy, Varghese(Vasile Goldis University Press, 2012)
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Abstract:
TThe invention of novel antibiotics and other bioactive microbial metabolites continues to be an
important aim in new drug discovery programmes. Actinomycetes have the potential to synthesize lots of diverse
biologically vigorous secondary metabolites and in the last decades actinomycetes became the most productive
source for antibiotics. Therefore in the present study we analyze the antibacterial activity of the actinomycetes
isolated from grassland soil samples of Tropical Montane forest. A total of 33 actinomycete strains isolated were
characterized and screened for antibacterial activities using well diffusion method against six specific pathogenic
organisms. Identification of the isolates revealed that the majority of them were belonging to Streptomycetes
followed by Nocardia, Micromonospora, Pseudonocardia, Streptosporangium, Nocardiopsis and
Saccharomonospora. Among the 33 isolates, Gr1 strain showed antagonistic activity against all checked
pathogens. Nine strains showed antibacaterial activity against Listeria, Vibrio cholera, Bacillus cereus,
Staphylococcus aureus and Salmonella typhi and only 2 strains (Gr1and Gr25) showed antagonism to E. coli.
The overall percentage of activity of actinomycetes isolates against each pathogenic bacterium was also
calculated. While 63.63% of the actinomycetes were antagoinistic against Listeria, Vibrio cholerae, and Bacillus
cereus, 60.6% of them were antagonistic to Staphylococcus aureus. Very few isolates (6.06%) showed
antibacterial activity against E. coli. In general most of the actinomycetes isolates were antagonistic to grampositive
bacteria such as Listeria, Bacillus and Staphylococcus than Gram-negative bacteria Vibrio cholerae, E.
coli and Salmonella
Description:
Studia Universitatis “Vasile Goldiş”, Seria Ştiinţele Vieţii
Vol. 22, issue 3, 2012, pp. 451-455