Eswar Shankar,P N; Anu, Joseph; Paulose,C S(Department of Biotechnology, May 17, 2006)
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Abstract:
In the present study dopamine was measured in the hypothalamus, brainstem, pancreatic islets and plasma, using HPLC. Dopamine D2 receptor
changes in the hypothalamus, brainstem and pancreatic islets were studied using [3H] YM-09151-2 in streptozotocin-induced diabetic and insulintreated
diabetic rats. There was a significant decrease in dopatnine content in the hypothalamus (P<0.001), brainstem (P<0.001), pancreatic islets
(P<0.001) and plasma (P<0.00I) in diabetic rats when compared to control. Scatchard analysis of [3H] YM-09151-2 in the hypothalamus of
diabetic rats showed a significant decrease in Bax (P<0.001) and Kd, showing an increased affinity of D2 receptors when compared to control.
Insulin treatment did not completely reverse the changes that occurred during diabetes. There was a significant decrease in B,nax (P<0.01) with
decreased affinity in the brainstem of diabetic rats. The islet membrane preparation of diabetic rats showed a significant decrease (P<0.001) in the
binding of [3H] YM-09 151-2 with decreased Kd (P<0.001) compared to control. The increase in affinity of D2 receptors in hypothalamus and
pancreatic islets and the decreased affinity in brainstem were confirmed by competition analysis. Thus our results suggest that the decreased
dopamine D, receptor function in the hypothalamus, brainstem and pancreas affects insulin secretion in diabetic rats, which has immense clinical
relevance to the management of diabetes.
Mohanan,Valiyaveettil; Balarama Kaimal,S; Paulose, Cheramadathikudyil S(Department of Bio Technology, August 30, 2004)
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Abstract:
The present study was to investigate the rote of central 5-11T and 5-HT,:v receptor Lindin4o
and acne expression in it 'at mo(lel of pancreatic regeneration using 60" -, pancreatcutumy.
The pancreatic regeneration was evaluated by 5-HT content and 5-HT,,receptor gene
expression in the cerebral cortex (CC) and brain stem MS) of Alain opcrate,t, 7 It utd
7 (.lays panereatectomised rats. 5-11T content significantly increased in the CC' (I' 1.1)11
and 13S (P 0.05) of 72 Ii p.ntcreateetomiscd rats. Sympathetic activity was decreased as
indicated by the significantly decreased norcpiuephrine (NIi) and epinephrine (FTI) Icvcl
(1' < 0.001 and P < 0.05) in the plasma of 72 h panereateetomised rats. 5-111 ,^, receptor
density and affinity was decreased in the CC (P < 0.01) and BS (P < 0.01). These rh:)nge;
correlated with a diminished 5-IITIA receptor mRNA expression in the brain region. studied.
Our resuils suggest that the brain 5-11T through 5-HTin receptor has it funcuon:0 rule
iii 11w pi+ncreatic regcner:ttion through the sympathetic regulation.
Mohanan,Valiyaveettil; Finla, Chathu; Paulose, Cheramadathikudyil S(Department of Bio Technology, December 1, 2004)
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Abstract:
The purpose of this study was to investigate the role of central 5-HT2C receptor binding in rat model of pancreatic regeneration
using 60-70% pancreatectomy. The 5-HT and 5-HT2c receptor kinetics were studied in cerebral cortex and brain stem of sham
operated, 72 h pancreatectomised and 7 days pancreatectomised rats. Scatchard analysis with [3H] mesulergine in cerebral
cortex showed a significant decrease (p < 0.05) in maximal binding (B^,ax) without any change in Kd in 72 h pancreatectomised
rats compared with sham. The decreased Bmax reversed to sham level by 7 days after pancreatectomy. In brain stem , Scatchard
analysis showed a significant decrease (p < 0.01) in Bax with a significant increase (p < 0.01) in Kd. Competition analysis
in brain stem showed a shift in affinity towards a low affinity. These parameters were reversed to sham level by 7 days after
pancreatectomy. Thus the results suggest that 5-HT through the 5-HT2C receptor in the brain has a functional regulatory role in
the pancreatic regeneration. (Mol Cell Biochem 272: 165-170, 2005)
Ani Das, V; Finla, Chathu; Paulose,C S(Department of Bio Technology, April 20, 2006)
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Abstract:
Sympathetic stimulation inhibits insulin secretion. a2-Adrenergic receptor is known to have a regulatory role in the sympathetic function. We
investigated the changes in the a2-adrenergic receptors in the brain stein and pancreatic islets using [3H]Yohimbine during pancreatic regeneration
in weanling rats. Brain stem and pancreatic islets of experimental rats showed a significant decrease (p<0.001) in norepinephrine (NE) content at
72 h after partial pancreatectomy. The epinephrine (EPI) content showed a significant decrease (p<0.001) in pancreatic islets while it was not
detected in brain stem at 72 h after partial pancreatectomy. Scatchard analysis of [3H]Yohimbine showed a significant decrease
(p<0.05) and Kd at 72 h after partial pancreatectomy in the brain stem. In the pancreatic islets, Scatchard analysis of [3H]Yohimbine showed a signiinfiBca'nnatx
decrease (p<0.001) in B,nax and Kd (p<0.05) at 72 h after partial pancreatectomy. The binding parameters reversed to near sham by 7 days after
pancreatectomy both in brain stein and pancreatic islets. This shows that pancreatic insulin secretion is influenced by central nervous system inputs
from the brain stem. In vitro studies with yohimbine showed that the a2-adrenergic receptors are inhibitory to islet DNA synthesis and insulin
secretion. Thus our results suggest that decreased a2-adrenergic receptors during pancreatic regeneration functionally regulate insulin secretion
and pancreatic 13-cell proliferation in weanling rats.
Paulose,C S; Peeyush, K T; Anju, T R(Springer Science+Business Media, LLC 2009, 2009)
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Abstract:
Hypoxia in neonates can lead to biochemical
and molecular alterations mediated through changes in
neurotransmitters resulting in permanent damage to brain.
In this study, we evaluated the changes in the receptor
status of GABAA in the cerebral cortex and brainstem of
hypoxic neonatal rats and hypoxic rats supplemented with
glucose and oxygen using binding assays and gene
expression of GABAAa1 and GABAAc5. In the cerebral
cortex and brainstem of hypoxic neonatal rats, a significant
decrease in GABAA receptors was observed, which
accounts for the respiratory inhibition. Hypoxic rats sup-
plemented with glucose alone and with glucose and oxygen
showed, respectively, a reversal of the GABAA receptors,
andGABAAa1 and GABAAc5 gene expression to control.
Glucose acts as an immediate energy source thereby
reducing the ATP-depletion-induced increase in GABA
and oxygenation, which helps in encountering anoxia.
Resuscitation with oxygen alone was less effective in
reversing the receptor alterations. Thus, the results of this
study suggest that reduction in the GABAA receptors
functional regulation during hypoxia plays an important
role in mediating the brain damage. Glucose alone and
glucose and oxygen supplementation to hypoxic neonatal
rats helps in protecting the brain from severe hypoxic
damage.
Balarama Kaimal,S; Gireesh,G; Paulose,C S(Department of Biotechnology, January 4, 2007)
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Abstract:
Gamma amino outyric acid is a major
inhibitory neurotrarsr titter in the central nervous
system. In the preset study sv, Have investigate(' the
alteration of GABA receptor, In t he hrain stem of rats
during pancreatic regeneration. Three groups of rats
were used for the study: sham operated, 72 It and
7 days partially pancreatectonnsea. GABA was (juan-
(ified by [H]GABA receptor iispiacement method.
GABA receptor kin: 10, pat at i et•ers were studied by
using the binding of F'.](iAhA as ligand to the Triton
X-100 treated me,i1,;-:mes a1,J displacement with
unlabelled GABA. GhRA,v receptor activity was
studied by using the [` -1 h3cuculline and displacement
with unlabellecV euculline. ;.\13A content significantly
decreased (1' < (1.(101 ) it, 0-e brain stern during
the regeneration of pancreas. 'I hl, high affinity (IAI3A
receptor binding sho?:ed it sigii'f cant decrease in 131„.,\
(P < 11.01) and K,I 1).05) n 72 h and 7 days after
partial pancreatee 'timv. ";:flhicuculline hin(Iing
showed it signih eat, 'le ( r(, :,e in /Jn1,s and K,I
(P < 0.001) in 72 h pa^.rcreaw,, mised rats when compared
with sham wt--tt' as P,n and K,I reversed to
near sham after 7 da,s of pancreatectomv. The results
sugge,) that GAB A throur,r; ('GABA receptors in
brain Atcem has a regulatory uie during active regeneration
of pancreas which will have inunense clinical
significance in the treatment of cliahetcs.
Ani Das, V; Savitha,B; Paulose,C S(Department of Biotechnology, April 4, 2006)
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Abstract:
purpose of this study was to investigate the
role of brain al-adrenergic receptor binding in the rat model
of pancreatic regeneration using 60-70% pancre:dectorny.
The a, -adrenergic receptors kinetics was studied in the
cerebral cor:cx and brain stem of sham operated . 72 It pan-
crea(ectoinised and 7 days pancreatectomised rats. Scar
chard analysis with I `I I lprazocin in cerebral cartes and brain
stein showed a significant decrease (/' < 0.01). (P < 0.05)
in maximal binding ( 1),,,,,) with it significant decrease
(P < 0.001 ), ( P < 0.01) in the K,,in 72 It pancreatecto-
raised rats compared with sham , respectively . Competition
analysis in cerebral cortex and brain stem showed it shift in
affinity during pancreatic regeneration . The sympathetic
activity was decreased as indicated by the significantly de-
increased norepinephrine level in the plasma (P < 0.001),
cerebral cortex (P < 0.01) and brain stem (P < 0.001) of
72 h pancreatectomised rats compared to sham . Thus, from
our results it is suggested that the central a, -adrenergic
receptors have a functional role in the pancreatic regenera-
Lion mediated through the sympathetic pathway.
Rajool Shanis, C P; Pillai, N G K(Cochin University Of Science And Technology, November , 2014)
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Abstract:
The present study is the first attempt to understand population characteristics of the deep-sea pandalid shrimp, P. quasigrandis and to assess the status of these resources off Kerala coast.Total mortality coefficient (Z) of P. quasigrandis estimated by various methods.Natural mortality coefficient (M) calculated was 0.65 and 1.02 by Pauly‟sempirical formula and Srinaths‟s formula respectively
The deep-sea shrimp P. quasigrandis exploited from the present fishing ground and their monetary return has started showing a declining trend. By observing the current yield and economic return, there is no further scope for increasing the catch from the present fishing ground. The study indicated that majority of the deep-sea shrimp trawlers, especially targeted for pandalid shrimps still concentrated off Kollam area (Quilon Bank). Even though researchers had located several potential deep-sea fishing grounds based on exploratory surveys in Indian EEZ , fishermen are unaware of these fishing grounds located and hence sharing the information about new potential deep-sea fishing grounds could avert the possible stock decline due to the intensive targeted deep-sea shrimp fishery in the Quilon Bank. Hence, the present
study recommended that part of the effort from existing fishing grounds may be shifted to newly located deep-sea fishing grounds which will help in a sustainableexploitation of deep-sea resources off Kerala coast.
Jayakrishnan, R; Dr.Vijayakumar, K P(Cochin University of Science & Technology, April , 2008)
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Abstract:
The present thesis can be divided into three areas:1) the
fabrication of a low temperature photo-luminescence and photoconductivity
measuring unit 2) photo-luminescence in the chalcopyrite
CulnSez and CulnS2 system for defect and composition analysis and
3) photo-luminescence and photo-conductivity of In:JS3. This thesis
shows that photo-luminescence is one of most essential semiconductor
characterization tool for a scientific group working on photovoltaics.
Tools which can be robust, non-destructive, requiring minimal sample
preparation for analysis and most informative of the device
applications are sought after by industries and this thesis is towards
establishing photo-luminescence as "THE" tool for semiconductor
characterization. The possible application of photo-luminescence as a
tool for compositional and quality analysis of semiconductor thin
films has been worked upon by this thesis. Photo-conductivity
complement photo-luminescence and together they provide all the
information required for the fabrication of an opto-electronic device.
Description:
Department of Physics,
Cochin University of Science and Technology
Unnikrishnan, K P; Jayan,Thomas; Nampoori, V P N; Vallabhan, C P G(Elsevier, June , 2002)
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Abstract:
Here we report measurements of third-order susceptibility χ(3), figure of merit F defined as χ(3)/α (where α is the absorption coefficient) and second hyperpolarizability 〈γ〉 of some metal substituted phthalocyanines and a naphthalocyanine in solutions of dimethyl formamide using degenerate four wave mixing at 532 nm under nanosecond excitation. It was found that among samples investigated, bis-naphthalocyanine possessed the highest value of 〈γ〉 followed by the bis-phthalocyanine. This observation is explained on the basis that bis-naphthalocyanine followed by bis-phthalocyanine has higher degree of π electron conjugation.
Rare earth exchanged Na–Y zeolites,
H-mordenite, K-10 montmorillonite clay and amorphous
silica-alumina were effectively employed for the
continuous synthesis of nitriles. Dehydration of benzaldoxime
and 4-methoxybenzaldoxime were carried
out on these catalysts at 473 K. Benzonitrile (dehydration
product) was obtained in near quantitative yield
with benzaldoxime whereas; 4-methoxybenzaldoxime
produces both Beckmann rearrangement (4-methoxyphenylformamide)
as well as dehydration products
(4-methoxy benzonitrile) in quantitative yields. The
production of benzonitrile was near quantitative under
heterogeneous reaction conditions. The optimal protocol
allows nitriles to be synthesized in good yields
through the dehydration of aldoximes. Time on stream
(TOS) studies show decline in the activity of the catalysts
due to neutralization of acid sites by the basic reactant
and product molecules and water formed during the
dehydration of aldoximes.
George, Sleeba; Dr.Meera Bai,M(Cochin University Of Science And Technology, January , 2004)
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Abstract:
The main objective of the Research was to study the Delayering
scheme implemented in FACT in all its aspects and to examine
wlietlier implementation of delayering has contributed to improvement in
organizational performance by improving managerial motivation and team
effectiveness. To enable this, a post-delayering impact assessment was
done by eliciting views and analyzing the perception of managers
affected by the change programme. Organisation-wide Surveys were
carried out as part of data collection - prior to, during and after implementation. The methodology adopted was the Case Study
approach, which involved a longitudinal study of the
Implementation process using tools such- as Observation, Opinion
surveys and Interviews and relating the research findings with the
theories/ studies on the subject for generalization and for drawing
conclusions
Description:
School of Management Studies
Cochin University of Science and Technology
Suresh, Vettoor V; Nandakumaran, V M(Physica C,Elsevier Science, 1992)
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Abstract:
The density of states and the low temperature specific heat of higb-Tc superconductors are calculated in a functional integral formalism using the slave boson technique. The manybody calculation in a saddle point approximation shows that the Iow energy
sector is dominated by 3 single band. The calculated values of density of states are in good agreement with experimental results.
Hatha, A A M; Krishna, M P; Rinoy, Varghese(Indian Society for Education and Environment, March , 2012)
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Abstract:
Soil microorganisms play a main part in organic matter decomposition and are consequently necessary to soil ecosystem
processes maintaining primary productivity of plants. In light of current concerns about the impact of cultivation and climate
change on biodiversity and ecosystem performance, it is vital to expand a complete understanding of the microbial community
ecology in our soils. In the present study we measured the depth wise profile of microbial load in relation with important soil
physicochemical characteristics (soil temperature, soil pH, moisture content, organic carbon and available NPK) of the soil
samples collected from Mahatma Gandhi University Campus, Kottayam (midland region of Kerala). Soil cores (30 cm deep)
were taken and the cores were separated into three 10-cm depths to examine depth wise distribution. In the present study,
bacterial load ranged from 141×105 to 271×105 CFU/g (10cm depth), from 80×105 to 131×105 CFU/g (20cm depth) and from
260×104 to 47×105 CFU/g (30cm depth). Fungal load varies from 124×103 to 27×104 CFU/g, from 61×103 to110×103 CFU/g
and from 16×103 to 49×103 CFU/g at 10, 20 and 30 cm respectively. Actinomycetes count ranged from 129×103 to 60×104
CFU/g (10cm), from 70×103 to 31×104 CFU/g (20cm) and from 14×103 to 66×103 CFU/g (30cm). The study revealed that there
was a significant difference in the depthwise distribution of microbial load and soil physico-chemical properties. Bacterial,
fungal and actinomycetes load showed a decreasing trend with increasing depth at all the sites. Except pH all other
physicochemical properties showed decreasing trend with increasing depth. The vertical profile of total microbial load was well
matched with the depthwise profiles of soil nutrients and organic carbon that is microbial load was highest at the soil surface
where organics and nutrients were highest
Description:
Indian J. Edu. Inf. Manage., Vol. 1, No. 3 (Mar 2012)
Saira Joseph; Dr. Binu Paul(Cochin University of Science and Technology, November 20, 2016)
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Abstract:
Over the past decade, there is a great demand for permission to transmit large bandwidth
concurrent with existing narrowband signals. In 2002, the federal communications
commission (FCC) decided to permit use of ultrawideband (UWB) systems.
UWB systems are unique in their large instantaneous bandwidth and potential for lowcost
digital design that enables a single system to operate in different modes as a
communication device, radar etc. UWB systems aim at covering the frequency band of
3.1-10.6 GHz defined by the FCC. The addition of more and more features in each new
generation communication system demands universal antennas suitable for operation in
multiple bands. In this regard, designing a multiband antenna which also covers the
UWB range without deteriorating the UWB performance is of great interest. Also, a
significant issue in communication systems is to miniaturize the antenna size while
providing good performance over the operation bands.
The thesis presents three different designs of CPW-fed multiband antennas. The first
design uses a fractal concept to achieve multiband operation. In the second and third
designs, a slotted circular monopole and a spiral monopole are used respectively for
multiband operation with UWB applications. For both these cases, variations from the
basic antenna geometry are also proposed in which the frequency of operation is
lowered without increase in overall antenna size. Design guidelines are developed for
all antennas to facilitate design on substrates of different permittivity and thickness. The
antennas are analyzed in both frequency and time domain for complete characterization