Madhu, K M; Dr.Chandrasekaran,M(Cochin University of Science and Technology, April , 2007)
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Abstract:
The beta-glucosidase enzyme purified from the marine fungus, Aspergillus
sydowii BTMFS 55 showed a good yield of enzyme production under solid state
fermentation. The statistical optimization of the media components revealed that
moisture content, concentration of peptone and inoculum are the major parameters
which supported the maximal enzyme production. The purified enzyme showed low
pH activity and stability, glucose tolerance and activation by ethanol. It could produce
ethanol from wheat bran and rice straw by simultaneous saccharification and
fermentation with yeast.The glucosidase purified from Aspergillus sydowii BTMFS 55
shows great potential for several biotechnological applications such as the production
of bio-ethanol from agricultural biomass and improvement in the aromatic character
of wines and fruit juices through the hydrolysis of flavour glucosidic precursors.
There is immense scope for the application of this marine fungus in the biofuel
production besides in other industries provided further studies are pursued in
exploiting this enzyme and the organism particularly scale up studies with respect to
application. There is also ample scope for cloning of the gene encoding beta-glucosidase
in domesticated hosts such as Pichia pastoris or S. cerevisiae that can produce ethanol
directly from cellulosic biomass.
Description:
Department of Biotechnology,
Cochin University of Science and Technology
Four species of bacteria which included Pseudomonas
fluorescens, Vibrio cho!erae and Vibrio costicola were
observed to produce glutaminase both as extracellular
and intracellular fractions. Comparatively both the fractions
were higher in mineral media supplemented with 1%
glutamine than in nutrient broth added with or without
glutamine. Extracellular glutaminase production was about
2.6-6.8 times greater than the intracellular production
by all the tested strains
Chandrasekaran, M; Rajeev Kumar, S; Sabu, A; Suresh, P V; Keerthi, T R(Kluwer Academic Publishers, August 6, 1999)
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Abstract:
Beauveria sp. BTMF S10 isolated from marine sediment produced extracellular L-glutaminase. Maximal L-
glutaminase yield (46.9 U/ml) was obtained in a medium supplemented with 1% (w/v) yeast extract and sorbitol,
9% (w/v) sodium chloride and 0.2% (w/v) methionine, initial pH 9.0 and at 27 °C after 108 h. This enzyme was
inducible and growth-associated.
Description:
World Journal of Microbiology & Biotechnology 15: 751±752, 1999.
Chandrasekaran, M; Madhu, K M; Beena, P S(Springer, 2009)
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Abstract:
A potential fungal strain producing extracellular β-glucosidase enzyme was isolated from sea water and identified as ^ëéÉêJ
Öáääìë=ëóÇçïáá BTMFS 55 by a molecular approach based on 28S rDNA sequence homology which showed 93% identity
with already reported sequences of ^ëéÉêÖáääìë=ëóÇçïáá in the GenBank. A sequential optimization strategy was used to enhance
the production of β-glucosidase under solid state fermentation (SSF) with wheat bran (WB) as the growth medium.
The two-level Plackett-Burman (PB) design was implemented to screen medium components that influence β-glucosidase
production and among the 11 variables, moisture content, inoculums, and peptone were identified as the most significant
factors for β-glucosidase production. The enzyme was purified by (NH4)2SO4 precipitation followed by ion exchange chromatography
on DEAE sepharose. The enzyme was a monomeric protein with a molecular weight of ~95 kDa as determined
by SDS-PAGE. It was optimally active at pH 5.0 and 50°C. It showed high affinity towards éNPG and enzyme has a hã and
sã~ñ of 0.67 mM and 83.3 U/mL, respectively. The enzyme was tolerant to glucose inhibition with a há of 17 mM. Low concentration
of alcohols (10%), especially ethanol, could activate the enzyme. A considerable level of ethanol could produce from
wheat bran and rice straw after 48 and 24 h, respectively, with the help of p~ÅÅÜ~êçãóÅÉë=ÅÉêÉîáëá~É in presence of cellulase
and the purified β-glucosidase of ^ëéÉêÖáääìë=ëóÇçïáá BTMFS 55.
Description:
Biotechnology and Bioprocess Engineering 2009, 14: 457-466
DOI/10.1007/s12257-008-0116-2
Anas, A; Bright Singh, I S(Centre for Fish Disease Diagnosis and Management, School of Environmental Studies, 2005)
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Abstract:
Present work is aimed at development of an appropriate microbial technology for protection of larvae of macrobrachium rosenbergii from disease and to increase survival rate in hatcheries. Application of immunostimulants to activate the immune system of cultured animals against pathogen is the widely accepted alternative to antibiotics in aquaculture. The most important immunostimulant is glucan. Therefore a research programme entitled as extraction of glucan from Acremonium diospyri and its application in macrobrachium rosenbergii larval rearing system along with bacterians as microspheres. The main objectives of the study are development of aquaculture grade glucan from acremonium diospyri, microencapsulated drug delivery system for the larvae of M. rosenbergii and microencapsulated glucan with bacterian preparation for the enhanced production of M. rosenbergii in larval rearing system. Based on the results of field trials microencapsulated glucan with bacterin preparation, it is concluded that the microencapsulated preparation at a concentration of 25g per million larvae once in seven days will enhance the production and quality seed of M. rosenbergii.
Rajesh, M; Dr.Nampoori,V P N(Cochin University of Science & Technology, July , 2006)
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Abstract:
The thesis presented the fabrication and characterisation of
polymer optical fibers in their applications as optical amplifier and smart sensors.Optical polymers such as PMMA are found to be a very good host material due
to their ability to incorporate very high concentration of optical gain media like
fluorescent dyes and rare earth compounds. High power and high gain optical
amplification in organic dye-doped polymer optical fibers is possible due to
extremely large emission cross sections of oyes. Dye doped (Rhodamine 6G)
optical fibers were fabricated by using indigenously developed polymer optical
fiber drawing tower. Loss characterization of drawn dye doped fibers was carried
out using side illumination technique. The advantage of the above technique is
that it is a nondestructive method and can also be used for studying the
uniformity in fiber diameter and doping. Sensitivity of the undoped polymer
fibers to temperature and microbending were also studied in its application in
smart sensors.Optical amplification studies using the dye doped polymer optical fibers were
carried out and found that an amplification of l8dB could be achieved using a
very short fiber of length lOcm. Studies were carried out in fibers with different
dye concentrations and diameter and it was observed that gain stability was
achieved at relatively high dye concentrations irrespective of the fiber diameter.Due to their large diameter, large numerical aperture, flexibility and geometrical
versatility of polymer optical fibers it has a wide range of applications in the field
of optical sensing. Just as in the case of conventional silica based fiber optic
sensors, sensing techniques like evanescent wave, grating and other intensity
modulation schemes can also be efficiently utilized in the case of POF based
sensors. Since polymer optical fibers have very low Young's modulus when
compared to glass fibers, it can be utilized for sensing mechanical stress and
strain efficiently in comparison with its counterpart. Fiber optic sensors have
proved themselves as efficient and reliable devices to sense various parameters
like aging, crack formation, weathering in civil structures. A similar type of study
was carried out to find the setting characteristics of cement paste used for
constructing civil structures. It was found that the measurements made by using
fiber optic sensors are far more superior than that carried out by conventional
methods. More over,POF based sensors were found to have more sensitivity as
well.
Description:
International School of Photonics,
Cochin University of Science & Technology
Samuel Varghese, K; Dr.Nampoori,V P N; Dr.Girijavallabhan,C P(Cochin University of Science and Technology, December , 2008)
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Abstract:
This Thesis deals with the fabrication and characterization of novel all-fiber components for access networks. All fiber components offer distinctive advantages due to low forward and backward losses, epoxy free optical path and high power handling. A novel fabrication method for monolithic 1x4 couplers, which are vital components in distributed passive optical networks, is realized. The fabrication method differs from conventional structures with a symmetric coupling profile and hence offers ultra wideband performance and easy process control. New structure for 1x4 couplers, by fusing five fibers is proposed to achieve high uniformity, which gives equivalent uniformity performance to 1x4 planar lightwave splitters, isolation in fused fiber WDM is improved with integration of long period gratings. Packaging techniques of fused couplers are analyzed for long term stability.
Description:
International School of Photonics, Cochin University of Science and Technology
Tintu, R; Dr. Sheenu, Thomas(Cochin University of Science and Technology, June , 2011)
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Abstract:
In this context,in search of new materials based on chalcogenide glasses,we have developed a novel technique for fabrication of chalcogenide nano composites which are presented in this theis.The techniques includes the dissolution of bulk chalcogenide glasses in amine solvent.This solution casting method allows to retain the attractive optical properties of chalcogenide glasses enabling new fabrication routes for realization of large area thick-thin films with less cost. Chalcogenide glass fiber geometry opens new possibilities for a large number of applications in optics,like remote temperature measurements ,CO2 laser power delivery, and optical sensing and single mode propagation of IR light.We have fabricated new optical polymer fibers doped with chalcogenide glasses which can be used for many optical applications.The present thesis also describes the structural,thermal and optical characterization of certain chalocogenide based materials prepared for different methods and its applications.
Description:
International School of Photonics, Cochin University of Science and Technology
Rajesh, Mandamparambil; Sheeba, Mavila; Geetha, Karinjamanna; Vallabhan, Chakkalakkal P G; Radhakrishnan, Padmanabhan; Nampoori, Vadakkedathu P N(Optical Society of America, January , 2007)
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Abstract:
The fabrication and characterization of a Rhodamine 6G-doped polymer optical fiber amplifier have been carried out. Two different schemes were employed to characterize the optical fiber: the stripe illumination technique to study the fiber as a gain medium and another technique to study its performance as an amplifier. We observed a spectral narrowing from 42 to 7 nm when the pump energy was increased to 6 mJ in the stripe illumination geometry. A gain of 18 dB was obtained in the amplifier configuration. The effects of pump power and dye concentration on the performance of the fiber as an amplifier were also studied.
Sheeba, M; Rajesh, M; Nampoori, V P N; Radhakrishnan, P(Optical Society of America, 2008)
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Abstract:
Rhodamine 6G and Rhodamine B dye mixture doped polymer optical fiber amplifier (POFA), which can operate in a broad wavelength region (60 nm), has been successfully fabricated and tested. Tunable operation of the amplifier over a broad wavelength region is achieved by mixing different ratios of the dyes. The dye doped POFA is pumped axially using 532 nm, 10 ns laser pulses from a frequency doubled Q-switched Nd: YAG laser and the signals are taken from an optical parametric oscillator. A maximum gain of 22.3 dB at 617 nm wavelength has been obtained for a 7 cm long dye mixture doped POFA. The effects of pump energy and length of the fiber on the performance of the fiber amplifier are also studied. There exists an optimum length for which the amplifier gain is at a maximum value.
Beena,Mary John; Sudha Kartha, C(Department of Physics, January , 2008)
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Abstract:
Holographic technology is at the dawn of quick evolution in various new
areas including holographic data storage, holographic optical elements, artificial
intelligence, optical interconnects, optical correlators, commerce, medical practice,
holographic weapon sight, night vision goggles and games etc. One of the major
obstacles for the success of holographic technology to a large extent is the lack of
suitable recording medium.
Compared with other holographic materials such as dichromated gelatin
and silver halide emulsions, photopolymers have the great advantage of recording
and reading holograms in real time and the spectral sensitivity could be easily
shifted to the type of recording laser used by simply changing the sensitizing dye.
Also these materials possess characteristics such as good light sensitivity, real
time image development, large dynamic range, good optical properties, format
flexibility, and low cost. This thesis describes the attempts made to fabricate
highly economic photopolymer films for various holographic applications.
In the present work, Poly (vinyl alcohol) (PVA) and poly (vinyl chloride)
(PVC) are selected as the host polymer matrices and methylene blue (MB) is used
as the photosensitizing dye. The films were fabricated using gravity settling
method. No chemical treatment or pre/post exposures were applied to the films.
As the outcome of the work, photopolymer films with more than 70% efficiency, a
permanent recording material which required no fixing process, a reusable
recording material etc. were fabricated.
Sreekumar, K(Optical society of America, June 20, 2004)
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Abstract:
A methylene-blue-sensitized polymer blend of polyvinyl alcohol and polyacrylic acid is fabricated and
tested for holographic recording. It was found to have good characteristics such as high sensitivity,
storage stability, ease of fabrication, and environmental stability. Optimization of the ratio of polyvinyl
alcohol polyacrylic acid, the sensitizer concentration, pH, energy, diffraction efficiency measurements,
etc., have been done. pH is found to have a great influence on the recovery of the dye in this matrix.
The results of experimental investigations into the properties of this new material are reported.
Sudha Kartha, C(Optical society of America, June 20, 2004)
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Abstract:
A methylene-blue-sensitized polymer blend of polyvinyl alcohol and polyacrylic acid is fabricated and
tested for holographic recording. It was found to have good characteristics such as high sensitivity,
storage stability, ease of fabrication, and environmental stability. Optimization of the ratio of polyvinyl
alcohol polyacrylic acid, the sensitizer concentration, pH, energy, diffraction efficiency measurements,
etc., have been done. pH is found to have a great influence on the recovery of the dye in this matrix.
The results of experimental investigations into the properties of this new material are reported.
Sheeba, M; Dr. Radhakrishnan, P(Cochin University of Science and Technology, May 22, 2008)
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Abstract:
Although the main application of optical fibers are in the field of
telecommunication, optical fiber based sensors of various designs are
becoming valuable devices for wide industrial applications. The
advantages of optical fiber-based sensors include high sensitivity,
insensitivity to electromagnetic radiation; spark free, light weight and
minimal intrusiveness due to their relatively small size and deployment in
harsh and hostile environments. It has been proved that POI-7 based
sensors can be employed to detect a great variety of parameters including
temperature, humidity, pressure, refractive index etc.
The proposed thesis presented in six chapters deals with the work carried
on dye doped and undoped POF for photonic device applications such as
amplifier, laser and sensor
Description:
International School of Photonics
Cochin University of Science and Technology
Varkey,K P; Dr.Vijayakumar, K P(COCHIN UNIVERSITY OF SCIENCE AND TECHNOLOGY, August , 1999)
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Abstract:
The study on the fabrication and characterization of spray pyrolysed cadmium sulphide homojunction solar cells. As an alternative to the conventional energy source, the PV technology has to be improved. Study about the factors affecting the performance of the existing solar cells and this will result in the enhancement of efficiency of the cells. At the same time it is equally important to have R&D works on developing new photovoltaic devices and processes which are less expensive for large scale production. CdS is an important binary compound semiconductor, which is very useful in the field of photovoltaics. It is very easy to prepare large area CdS thin films. In order to fabricate thin film homojunction cadmium sulphide cells, prepared and characterized SnO2 thin film as the lower electrode, p-CdS as the active layer and n-CdS as window layer. Cadmium material used for the fabrication of homojunction solar cells is highly toxic. The major damage due to continued exposure to low levels of cadmium are on the kidneys, lungs and bones. The real advantage of spray pyrolysis process is that there is no emission of any toxic gases during the deposition. Very low concentration of the chemicals is needed in this process. The risk involved from this material is very low, though they are toxic. On large scale usage it may become necessary that the cells after their life, should be bought back by the companies to retrieve chemicals like cadmium. This will reduce environmental problem and also the material wastage
Sebastian, P J; Dr.Sathianandan, K(Cochin University Of Science And Technology, November 25, 1981)
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Abstract:
Eventhough a large number of schemes have been proposed and develoned for N9 laser ouined dye lasers the
relatively low efficiency compelled the scientists to
device new methods to improve the system efficiencs.
Energy transfer mechanism has been shown to he a convenien
tool for the enhancement of efficiency of dye lasers. Th
p resent work covers a detailed study of the performance
characteristics of a N2 laser pumped dye laser in the con—
ventional mode and also, when pumped by the energy transfer
mechanism. For .th.e present investigations a dye laser
pumped by a'N2 laser (A4200 kw peak power) was fabricated.
The grating at grazing incidence was used as the beam expanding
device; A t its best performance the system was
giving an output peak power of l5 kW for a 5 X lC"3H/l Rh—€
solution in methanol. T he conversion efficiency was 7.5;
The output beam was having 3 divergence of 2 mrad and bandwidth
o.9 A. Suitable modifications were suggested for
obtaining better conversion efficiency and bandwidth.
Description:
Department of Physics,
Cochin University Of Science And Technology
Jayaraj, M K; Dr.Girijavallabhan,C P(Cochin University of Science and Technology, June 20, 1988)
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Abstract:
This thesis contains the author's work in preparing efficient EL phosphors, the details of fabrication of low voltage operated thin film EL (TFEL) devices and DC TFEL devices. Some of the important work presented here are related to the white light emitting ZnS:Cu,Pr,Cl phosphor which can be colour tuned by changing the excitation frequency, observation of energy transfer from Cu/Ag ions to rare earth ions in ZnS:(Cu/Ag), RE,Cl phosphors, development of TFEL device which can be operated below 50V, optimization of the device parameters for long life, high brightness in terms of the active and insulating layer thicknesses, observation of dependence of threshold voltage for the onset of emission on frequency of excitation when a novel dielectric Eu2O3 film was used as insulator and the devices with multicolor emission using ZnS doped with rare earth as active layer. Characterization based on other devices based on ZnS:Sm, ZnS:Pr, ZnS:Dy and their emission characteristics are also illustrated
Description:
Department of Physics, Cochin University of Science and Technology